一种使用锌-锌试剂指示剂测定血清钙的新比色法。

A new colorimetric method for the measurement of serum calcium using a zinc-zincon indicator.

作者信息

Corns C M

机构信息

Department of Chemical Pathology, Middlesex Hospital Medical School, London, UK.

出版信息

Ann Clin Biochem. 1987 Nov;24 ( Pt 6):591-7. doi: 10.1177/000456328702400607.

DOI:10.1177/000456328702400607

PMID:3122631

Abstract

A new method is described for the measurement of serum calcium. Calcium in the sample displaces zinc from an EGTA complex, the zinc then forming a blue-coloured complex with a zincon indicator. The method is rapid, sensitive and linear; it may be applied directly to serum and is readily automated. No compound tested gave rise to any interference at physiological concentration, although excessive haemolysis leads to low results being obtained. To avoid interference from endogenous zinc a serum blank is required for each sample; this also avoids errors introduced by turbidity of the sample. The results obtained correlate well with atomic absorption spectroscopy (r = 0.935, y = 1.12x-0.28, n = 68).

摘要

本文描述了一种测定血清钙的新方法。样品中的钙从乙二醇双（2-氨基乙基醚）四乙酸（EGTA）络合物中置换出锌，然后锌与锌试剂指示剂形成蓝色络合物。该方法快速、灵敏且呈线性；可直接应用于血清，且易于自动化操作。尽管过度溶血会导致结果偏低，但在生理浓度下，所测试的任何化合物均未产生干扰。为避免内源性锌的干扰，每个样品都需要一个血清空白对照；这也避免了样品浑浊所引入的误差。所获得的结果与原子吸收光谱法相关性良好（r = 0.935，y = 1.12x - 0.28，n = 68）。