使用商业检测方法发现感染多种丙型肝炎病毒基因型后，应使用下一代测序技术进行确认。
Infection with multiple hepatitis C virus genotypes detected using commercial tests should be confirmed using next generation sequencing.
机构信息
Servicio de Microbiologia, Hospital Universitario de La Princesa; Instituto de Investigacion Sanitaria La Princesa, Madrid, Spain.
Unidad de Gestión Clinica de Microbiologia, Hospital Universitario San Cecilio; Instituto de Investigación Biosanitaria ibs.Granada; Red de Investigación en SIDA, RD16/0025/0040, Granada, Spain.
出版信息
Sci Rep. 2019 Jun 25;9(1):9264. doi: 10.1038/s41598-019-42605-z.
Current HCV genotyping methods may have some limitations in detecting mixed infections. We aimed to determine the accuracy of genotyping and the detection of mixed-genotype infections using the Abbott-RealTime HCV Genotype II assay (Abbott-RT-PCR) in comparison with a Roche-Next Generation Sequencing assay (Roche-NGS). Plasma samples collected from 139 HCV-infected patients tested with Abbott-RT-PCR, 114 with single genotype (GT) and 25 with mixed GTs were genotyped using Roche-NGS. Roche-NGS confirmed all single GTs obtained with Abbott-RT-PCR. One case of Abbott GT 4 was found as GT 1a using Roche-NGS. Genotype 5 was confirmed using Roche-NGS in 75% cases (3 out of 4 cases). Twenty-five patients were identified as having mixed HCVinfections using Abbott-RT-PCR. The concordance between Abbott-RT-PCR and Roche-NGS was 76% (19 out of 25 cases). Three mixed-GT infections identified with the Abbott assay (two (1b + 4); one (1a + 3)) were reported as pure 1b using Roche-NGS. Very divergent results were found for the other three samples. When compared to Roche-NGS, Abbott-RT-PCR has performed excellently for the determination of patients infected with single GTs. For patients that are categorized as having a mixed infection using Abbott-RT-PCR, we recommend an NGS assay as a confirmation test.
目前的 HCV 基因分型方法在检测混合感染时可能存在一些局限性。我们旨在比较 Abbott-RealTime HCV Genotype II 测定法（Abbott-RT-PCR）和罗氏下一代测序测定法（Roche-NGS），确定基因分型和检测混合基因型感染的准确性。对 139 例 HCV 感染患者的血浆样本进行 Abbott-RT-PCR 检测，114 例为单一基因型（GT），25 例为混合 GT。罗氏 NGS 对所有用 Abbott-RT-PCR 获得的单一 GT 进行了基因分型。罗氏 NGS 发现 1 例 Abbott GT4 为 GT1a。罗氏 NGS 证实了 75%（4 例中的 3 例）病例的基因型 5。25 例患者经 Abbott-RT-PCR 鉴定为混合 HCV 感染。Abbott-RT-PCR 与 Roche-NGS 的一致性为 76%（25 例中的 19 例）。Abbott 检测法鉴定的 3 例混合 GT 感染（2 例（1b + 4）；1 例（1a + 3））用罗氏 NGS 报告为纯 1b。另外三个样本的结果差异很大。与 Roche-NGS 相比，Abbott-RT-PCR 在确定单一 GT 感染患者方面表现出色。对于 Abbott-RT-PCR 分类为混合感染的患者，我们建议进行 NGS 检测作为确认试验。
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