钙离子载体A23187诱导猪甲状腺细胞胞质游离钙升高及碘释放：用发光蛋白测量胞质游离钙

Ca2+ ionophore A23187-induced rise in cytoplasmic free calcium and iodide discharge in porcine thyroid cells: measurement of cytoplasmic free calcium by aequorin.

作者信息

Takasu N, Murakami M, Nagasawa Y, Yamada T, Shimizu Y, Kojima I, Ogata E

机构信息

Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Nagano-ken, Japan.

出版信息

J Endocrinol. 1987 Dec;115(3):477-80. doi: 10.1677/joe.0.1150477.

DOI:10.1677/joe.0.1150477

PMID:3127512

Abstract

The cytoplasmic concentration of free calcium was measured using aequorin, a calcium-sensitive photoprotein. The Ca2+ ionophore A23187 induced a rise in cytoplasmic free calcium and iodide discharge in cultured porcine thyroid cells. The minimum dose of A23187 effecting an increase in cytoplasmic free calcium induced iodide discharge. The A23187-induced rise in cytoplasmic free calcium was followed by iodide discharge. The results indicate that A23187-induced iodide discharge is mediated by a rise in the cytoplasmic concentration of free calcium.

摘要

使用水母发光蛋白（一种对钙敏感的光蛋白）测量游离钙的细胞质浓度。Ca2+离子载体A23187可诱导培养的猪甲状腺细胞中细胞质游离钙升高和碘化物释放。引起细胞质游离钙增加的A23187的最小剂量可诱导碘化物释放。A23187诱导的细胞质游离钙升高之后是碘化物释放。结果表明，A23187诱导的碘化物释放是由细胞质游离钙浓度升高介导的。