College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China.
Bioresour Technol. 2019 Nov;291:121815. doi: 10.1016/j.biortech.2019.121815. Epub 2019 Jul 16.
In this study, mechanism of nitrogen transformation was investigated in terms of genetic associations (nitrogen-related gene groups) in co-composting of cattle manure and rice straw. Mutual validation among KEGG, Pearson correlation, stepwise regression, and Path analyses indicated that the functional genes synergistically affected on nitrogen transformation in composting process. NxrA/qnorB (0.9419 ± 0.0334) and (amoA + anammox)/Bacteria (0.7187 ± 0.0334) were the key functional gene groups mediating NH-N transformation. AmoA/(narG + napA) (-0.8400 ± 0.0129), amoA/bacteria (0.8692 ± 0.0273), and (nirK + nirS)/nosZ (1.1652 ± 0.0089) determined NO-N, NO-N and NO transformation, respectively. AmoA/(napA + narG) mediated both NO-N and NO-N transformation. AmoA/anammox (-0.7172 ± 0.0591) and (nirK + nirS)/nosZ (-0.6626 ± 0.0825) served as predominant factors for total nitrogen removal. These results provided a molecular-level insight that nitrification, anaerobic ammonia oxidation and denitrification (SNAD) might simultaneously contribute to nitrogen transformation during composting, rather than sequentially.
在这项研究中，我们从牛粪和稻草共堆肥中氮相关基因（氮相关基因群）的遗传关联的角度研究了氮转化的机制。KEGG、皮尔逊相关性、逐步回归和路径分析相互验证表明，功能基因在堆肥过程中协同影响氮转化。NxrA/qnorB（0.9419±0.0334）和（amoA+anammox）/Bacteria（0.7187±0.0334）是介导 NH-N 转化的关键功能基因群。AmoA/（narG+napA）（-0.8400±0.0129）、amoA/bacteria（0.8692±0.0273）和（nirK+nirS）/nosZ（1.1652±0.0089）分别决定了 NO-N、NO-N 和 NO 转化。AmoA/（napA+narG）介导了 NO-N 和 NO-N 的转化。AmoA/anammox（-0.7172±0.0591）和（nirK+nirS）/nosZ（-0.6626±0.0825）是总氮去除的主要因素。这些结果从分子水平上提供了一个见解，即硝化作用、厌氧氨氧化和反硝化作用（SNAD）可能在堆肥过程中同时而不是顺序地促进氮转化。
