School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Molecules. 2019 Jul 26;24(15):2727. doi: 10.3390/molecules24152727.
Psoriasis is a recurrent skin disease described as keratinocyte hyperproliferation and aberrant differentiation. Erianin, a bibenzyl compound extracted from , has displayed antitumor and anti-angiogenesis effects. However, the effects of erianin on a human keratinocyte cell line (HaCaT) are not fully understood. In the present study, we explored the effect of erianin on proliferation and apoptosis in HaCaT cells. Our results indicated that treatment with erianin ranging from 12.5 nM to 50 nM inhibited proliferation and induced apoptosis of HaCaT cells. In addition, erianin-induced apoptosis was accompanied by elevated reactive oxygen species (ROS). The ROS scavenger -acetyl-cysteine (NAC) attenuated this elevation. Moreover, treatment with erianin induced activation of the c-Jun N-terminal kinase (JNK)/c-Jun signaling pathway and suppressed the protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway, while pretreatment with NAC also reversed these effects. Collectively, these data demonstrated that erianin inhibited proliferation and induced apoptosis of HaCaT cells through ROS-mediated JNK/c-Jun and AKT/mTOR signaling pathways. Erianin could be recognized as a potential anti-psoriasis drug.
银屑病是一种反复发作的皮肤疾病，其特征为角质形成细胞过度增殖和分化异常。白皮杉醇是一种从 中提取的双苄基化合物，具有抗肿瘤和抗血管生成作用。然而，白皮杉醇对人角质形成细胞系（HaCaT）的影响尚不完全清楚。在本研究中，我们探讨了白皮杉醇对 HaCaT 细胞增殖和凋亡的影响。结果表明，12.5 nM 至 50 nM 的白皮杉醇处理抑制了 HaCaT 细胞的增殖并诱导其凋亡。此外，白皮杉醇诱导的凋亡伴随着活性氧（ROS）的升高。ROS 清除剂 N-乙酰半胱氨酸（NAC）减弱了这种升高。此外，白皮杉醇处理诱导 c-Jun N-末端激酶（JNK）/c-Jun 信号通路的激活，并抑制蛋白激酶 B（AKT）/哺乳动物雷帕霉素靶蛋白（mTOR）信号通路，而 NAC 的预处理也逆转了这些作用。综上所述，这些数据表明，白皮杉醇通过 ROS 介导的 JNK/c-Jun 和 AKT/mTOR 信号通路抑制 HaCaT 细胞的增殖并诱导其凋亡。白皮杉醇可被视为一种有潜力的抗银屑病药物。
