Zhang Jing, Li Qing-Ru, Zhang Ming-Hao, You Ying, Wang Yu, Wang Yu-Hua
College of Food science and Engineering, Jilin Agricultural University, Changchun, China.
National Processing Laboratory for Soybean Industry and Technology, Changchun, China.
Biosci Biotechnol Biochem. 2019 Dec;83(12):2375-2385. doi: 10.1080/09168451.2019.1650633. Epub 2019 Aug 8.
Carotenoids have good biological activity in antioxidant, anti-aging and scavenging harmful free radicals. In this study, we screened a strain that produced carotenoids, and selected a stress condition which significantly improved carotenoids content. The strain was identified as PR106. Active oxygen generator TiO was the most significant factor to the carotenoids content of the . The content of carotenoids was 54.45 mg/g at 500 mg/L TiO, which was about 1.25 times of the control and the proportion of carotenoids also changed from 1:9:16 to 1:8.5:12. Further, we determined the reactive oxygen species (ROS) in YEPD medium and , found that the ROS (HO, O, and HO•) was significantly increased at 500 mg/L TiO in YEPD medium compared with the control, but increased in under 1000 mg/L TiO treated. These results suggested that the increase in carotenoids was related to ROS in .
类胡萝卜素在抗氧化、抗衰老和清除有害自由基方面具有良好的生物活性。在本研究中，我们筛选出了一株产类胡萝卜素的菌株，并选择了一种能显著提高类胡萝卜素含量的胁迫条件。该菌株被鉴定为PR106。活性氧发生器TiO对该菌株类胡萝卜素含量的影响最为显著。在500 mg/L TiO条件下，类胡萝卜素含量为54.45 mg/g，约为对照的1.25倍，类胡萝卜素比例也从1:9:16变为1:8.5:12。此外，我们测定了YEPD培养基中的活性氧（ROS），发现与对照相比，在500 mg/L TiO的YEPD培养基中ROS（HO、O和HO•）显著增加，但在1000 mg/L TiO处理下的[具体物质未明确]中ROS增加。这些结果表明，类胡萝卜素的增加与[具体物质未明确]中的ROS有关。
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