Laboratory of Experimental Pathology, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador, Bahia 40296-710, Brazil.
Laboratory of Cellular and Molecular Genetics, ICB, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901, Brazil.
Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.AME-0008-2019.
The MinION sequencer was launched by the Oxford Nanopore Technologies start-up as a disruptive technology for genome sequencing based on single-molecule synthesis. Its characteristics as a portable device, low cost, and simple library preparation have made it a good candidate for field researchers. MinION has been used to sequence a number of microorganisms, such as bacteria, viruses, and fungi. Based on the experience that characterized the Ebola virus genetic diversity in Guinea during the 2014-2015 outbreak, the ZiBRA (Zika in Brazil Real-time Analysis) project aimed to sequence a large number of Zika virus genomes during a mobile laboratory trip in northeast Brazil to provide important epidemiological information about the spread of this disease in this country. In response to the positive and rapid results obtained by the ZiBRA project, the Brazilian Ministry of Health and many leading institutions, such as the Pan American Health Organization and WHO, have shown interest in expanding the strategy used in this project to other countries dealing with arbovirus infection. *This article is part of a curated collection.
牛津纳米孔技术初创公司推出的 MinION 测序仪是一种基于单分子合成的基因组测序的颠覆性技术。它作为一种便携式设备、低成本和简单的文库制备的特点，使其成为现场研究人员的良好候选者。MinION 已被用于测序多种微生物，如细菌、病毒和真菌。基于在 2014-2015 年埃博拉病毒爆发期间描述几内亚病毒遗传多样性的经验，ZiBRA（巴西寨卡实时分析）项目旨在通过在巴西东北部的移动实验室旅行中对大量寨卡病毒基因组进行测序，为该疾病在该国的传播提供重要的流行病学信息。针对 ZiBRA 项目获得的积极和快速结果，巴西卫生部和许多主要机构，如泛美卫生组织和世界卫生组织，表现出了将该项目中使用的策略扩展到其他处理虫媒病毒感染的国家的兴趣。*本文是精选集的一部分。
