.的竞争适应性。 （你提供的原文不完整，翻译可能不太准确，建议补充完整原文内容以便更精准翻译）
Competitive fitness of .
作者信息
Kodio Ousmane, Georges Togo Antieme Combo, Sadio Sarro Yeya Dit, Fane Bintou, Diallo Fatimata, Somboro Amadou, Degoga Boureima, Kone Mahamadou, Coulibaly Gagni, Tolofoudje Mohamed, Bane Sidy, Sanogo Moumine, Kone Bourahima, Coulibaly Nadie, Dabitao Djeneba, Baya Bocar, Maiga Mamoudou, Bougoudogo Flabou, Samake Fasse, Dao Sounkalo, Doumbia Seydou, Diallo Souleymane, Diarra Bassirou
机构信息
University Clinical Research Center, University of Sciences, Techniques and Technologies of Bamako; National Health Laboratory, Ministry of Health and Social Affairs, Bamako, Mali.
University Clinical Research Center, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali.
出版信息
Int J Mycobacteriol. 2019 Jul-Sep;8(3):287-291. doi: 10.4103/ijmy.ijmy_97_19.
BACKGROUND
While, bacteria resistance mutations can affect competitive fitness, given our multidrug-resistant (MDR) prevalence, we conducted this study to determine the impact of MDR on the competitive fitness of Mycobacterium tuberculosis (MTB) complex MDR strains. We conducted a cross-sectional study at the University Clinical Research Center (UCRC) from January to December 2017. New TB patients over aged of 18 were recruited at University teaching hospital and health reference centers of Bamako in USTTB Ethical committee approved protocols.
METHODS
MDR and drug-susceptible (wild-type [WT]) MTB strains (T1 and Beijing) and MTB H37Rv were competed on solid media in UCRC's Tuberculosis Laboratory. Competitive and individual cultures were incubated for 14 days at 37°C with 7% CO2. Number of generation, generation time, and relative competitive fitness (W) of the strains were calculated. Data were analyzed with Epi-Info 7.1.5.2 software (CDC). P value was considered significant when it was <0.05. Scientific calculator (CS-82TL) was used for competitive fitness parameters calculations.
RESULTS
We performed 24 competitive cultures and 10 individual cultures. In individual cultures, strains' generation number was for Beijing (WT: 4.60 and mutant MR: 4.40), T1 (WT: 2.69 and MR: 2.37), and H37Rv: 2.91. Generation number of WT strains was less than those of MDR strains in both individual and competitive culture. Relative competitive fitness was below 1 (W<1) in 83.3%.
CONCLUSION
MDR strains were less competitive than WT strains in 83.3% of cases. Resistant mutation impacts bacteria fitness.
背景
虽然细菌耐药性突变会影响竞争适应性，但鉴于我们的多重耐药（MDR）患病率，我们开展了这项研究，以确定MDR对结核分枝杆菌（MTB）复合群MDR菌株竞争适应性的影响。2017年1月至12月，我们在大学临床研究中心（UCRC）进行了一项横断面研究。18岁以上的新结核病患者在巴马科大学教学医院和卫生参考中心招募，符合USTTB伦理委员会批准的方案。
方法
MDR和药物敏感（野生型[WT]）MTB菌株（T1和北京株）以及MTB H37Rv在UCRC结核病实验室的固体培养基上进行竞争。竞争培养物和单独培养物在37°C、7%二氧化碳条件下孵育14天。计算菌株的代数、世代时间和相对竞争适应性（W）。数据用Epi-Info 7.1.5.2软件（美国疾病控制与预防中心）进行分析。P值<0.05时被认为具有统计学意义。使用科学计算器（CS-82TL）计算竞争适应性参数。
结果
我们进行了24次竞争培养和10次单独培养。在单独培养中，北京株（WT：4.60，突变型MR：4.40）、T1株（WT：2.69，MR：2.37）和H37Rv的菌株代数分别为4.60、4.40、2.69、2.37和2.91。在单独培养和竞争培养中，WT菌株的代数均低于MDR菌株。83.3%的相对竞争适应性低于1（W<1）。
结论
83.3%的病例中，MDR菌株的竞争力低于WT菌株。耐药突变影响细菌适应性。
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