Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Murcia, Spain.
SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Castilla-La Mancha (UCLM)-Junta de Comunidades de Castilla-La Mancha (JCCM), Ciudad Real, Spain.
Transbound Emerg Dis. 2020 Jan;67(1):318-327. doi: 10.1111/tbed.13354. Epub 2019 Sep 26.
The objective of this study was to identify changes in serum proteome in dogs that may occur after an experimental infection at subclinical and clinical stages of canine leishmaniosis (CanL). For this purpose, canine pre- and post-infection with Leishmania infantum serum proteomes in the same dogs were analysed by a high-throughput label-based quantitative LC-MS/MS proteomic approach. A total of 169 proteins were identified, and 74 of them including complement C8 alpha chain, adiponectin, transferrin, sphingomyelin phosphodiesterase acid-like 3A and immunoglobulins showed different modulation between the different stages of CanL. These proteins could be considered as potential serum biomarkers of early diagnostic or disease progression in CanL. Additionally, biological pathways modulated during CanL such as blood coagulation or gonadotropin-releasing hormone receptor were revealed, which could help to understand the pathological mechanisms of the disease.
本研究的目的是鉴定在犬利什曼病（CanL）亚临床和临床阶段的实验感染后，可能发生在血清蛋白质组中的变化。为此，通过高通量基于标签的定量 LC-MS/MS 蛋白质组学方法分析了犬在感染前和感染后的血清蛋白质组。共鉴定出 169 种蛋白质，其中包括补体 C8 链、脂联素、转铁蛋白、鞘磷脂磷酸二酯酶酸样 3A 和免疫球蛋白，它们在 CanL 的不同阶段表现出不同的调节。这些蛋白质可以被认为是 CanL 早期诊断或疾病进展的潜在血清生物标志物。此外，还揭示了在 CanL 期间调节的生物学途径，如血液凝固或促性腺激素释放激素受体，这有助于理解疾病的病理机制。
