墨西哥犬埃立克体感染：流行率、发病率、合并感染及相关因素。
Ehrlichia canis in dogs of Mexico: Prevalence, incidence, co-infection and factors associated.
机构信息
Campus de Ciencias Biológicas y Agropecuarias, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
Campus de Ciencias Biológicas y Agropecuarias, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
出版信息
Comp Immunol Microbiol Infect Dis. 2019 Dec;67:101351. doi: 10.1016/j.cimid.2019.101351. Epub 2019 Sep 5.
Rickettsial infections in dogs of Mexico were investigated. A total of 246 dogs were blood sampled and initially screened to detect Ehrlichia canis, E. chaffeensis, E. ewingii, Anaplasma phagocytophilum and Rickettsia rickettsii by a quantitative real-time PCR (qPCR) assay. Sixty-five dogs were monitored and sampled twice 7-8 months apart. Using the qPCR, 72 positive dogs to E. canis were detected (prevalence of 29.26%). These dogs were also tested by nested PCR to detect the same pathogens. None of the studied dogs were positive to E. chaffeensis, E. ewingii, R. rickettsii nor A. phagocytophilum by both PCR assays. The cumulative incidence of E. canis infection was 38.46%. Sequencing analysis of the nested PCR products revealed 100% and 98.1% identity of E. canis and R. parkeri, respectively. We found a dog co-infected with E. canis and R. parkeri.
对墨西哥犬的立克次体感染进行了调查。共采集了 246 只狗的血液样本，并通过实时定量 PCR(qPCR) 检测初步筛选出犬埃立克体、查菲埃立克体、埃氏埃立克体、嗜吞噬细胞无形体和犬无形体，65 只狗被监测并在 7-8 个月后两次采样。通过 qPCR 检测到 72 只犬埃立克体阳性（29.26%的患病率）。这些狗还通过巢式 PCR 检测到相同的病原体。通过两种 PCR 检测，均未发现研究犬对查菲埃立克体、埃氏埃立克体、无形体或嗜吞噬细胞无形体呈阳性。犬埃立克体感染的累积发病率为 38.46%。巢式 PCR 产物的测序分析显示，犬埃立克体和犬埃立克体帕克立克体的同源性分别为 100%和 98.1%。我们发现一只犬同时感染了犬埃立克体和犬埃立克体帕克立克体。
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