一种基于荧光的方便检测蔗糖转运的方法，并将马铃薯中的蔗糖转运蛋白导入 菌株。
A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Strains.
机构信息
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, China.
National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, China.
出版信息
Molecules. 2019 Sep 26;24(19):3495. doi: 10.3390/molecules24193495.
A convenient and effective sucrose transport assay for strains is needed. Traditional methods, such as C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into , and a fluorescence assay based on esculin was used for the analysis of sucrose transport in strains. This showed that the heterologously expressed potato sucrose transporter is functional in . Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in .
需要一种方便有效的蔗糖转运分析方法来研究 菌株。传统方法，如 C-蔗糖同位素标记，使用放射性物质，不适合许多实验室。本文将马铃薯蔗糖转运蛋白导入 中，并利用表儿茶素荧光分析法分析 菌株的蔗糖转运。结果表明，异源表达的马铃薯蔗糖转运蛋白在 中具有功能。高效蔗糖转运的重组工程将有助于 菌株的蔗糖发酵。本文描述的测定方法为蔗糖转运蛋白在异源表达后的功能研究提供了重要的技术平台。
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