Department of Experimental and Clinical Pharmacology, Pomeranian Medical University, ul. Powstancow Wlkp 72, 70-111, Szczecin, Poland.
Department of Clinical Pharmacology, University Medicine of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, Germany.
Int J Mol Sci. 2019 Sep 28;20(19):4825. doi: 10.3390/ijms20194825.
Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC ( = 6) and SLC ( = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC-MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of , , , , , , , , , , , , and were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics.
唾液腺具有分泌功能，包括分泌外源性物质，其中包括药物。然而，目前尚无使用可靠蛋白定量方法测量药物转运体蛋白丰度的相关报道。因此，本研究采用 qRT-PCR 和液相色谱-串联质谱（LC-MS/MS）法，研究了人腮腺中临床相关 ABC（=6）和 SLC（=15）家族成员转运体的 mRNA 表达和绝对蛋白含量。几乎所有测定蛋白的丰度范围在 0.04 到 0.45 pmol/mg（OCT3＞MRP1＞PEPT2＞MRP4＞MATE1＞BCRP）之间。未检测到 、 、 、 、 、 、 、 、 、 、 和 的 mRNA。本研究首次提供了人腮腺中膜转运体蛋白丰度的相关信息，这可进一步用于定义内源性化合物和外源性物质的唾液双向转运（吸收和分泌）机制。
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