用磷酸钙-DNA共沉淀物转染哺乳动物细胞
Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates.
作者信息
Kumar Priti, Nagarajan Arvindhan, Uchil Pradeep D
出版信息
Cold Spring Harb Protoc. 2019 Oct 1;2019(10):2019/10/pdb.top096255. doi: 10.1101/pdb.top096255.
PMID:31575800
Abstract
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured cells. Here, we briefly review the use of calcium phosphate-DNA coprecipitates for transfection.
摘要
生化转染方法，包括磷酸钙介导的转染和二乙氨基乙基（DEAE）-葡聚糖介导的转染，多年来一直用于将核酸导入培养细胞。在此，我们简要回顾磷酸钙-DNA共沉淀物在转染中的应用。
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