间歇运动可降低糖尿病前期患者循环中 CD105 外泌体的水平。
Interval Exercise Lowers Circulating CD105 Extracellular Vesicles in Prediabetes.
机构信息
Department of Kinesiology, University of Virginia, Charlottesville, VA.
Division of Endocrinology and Metabolism, University of Virginia, Charlottesville, VA.
出版信息
Med Sci Sports Exerc. 2020 Mar;52(3):729-735. doi: 10.1249/MSS.0000000000002185.
BACKGROUND
Extracellular vesicles (EV) are purported to mediate type 2 diabetes and CVD risk and development. Physical activity and a balanced diet reduce disease risk, but no study has tested the hypothesis that short-term interval (INT) training would reduce EV compared with continuous (CONT) exercise in adults with prediabetes.
METHODS
Eighteen obese adults (age, 63.8 ± 1.5 yr; body mass index, 31.0 ± 1.3 kg·m) were screened for prediabetes using American Diabetes Association criteria (75 g oral glucose tolerance test). Subjects were randomized to INT (n = 10, alternating 3-min intervals at 90% and 50% HRpeak, respectively) or CONT (n = 8, 70% HRpeak) training for 12 supervised sessions over 13 d for 60 min·d. Cardiorespiratory fitness (V˙ O2peak), weight (kg), as well as ad libitum dietary intake were assessed and arterial stiffness (augmentation index via applanation tonometry) was calculated using total AUC during a 75-g oral glucose tolerance test performed 24 h after the last exercise bout. Total EV, platelet EV (CD31/CD41), endothelial EV (CD105; CD31/ CD41), platelet endothelial cell adhesion molecule (PECAM) (CD31), and leukocyte EV (CD45; CD45/CD41) were analyzed via imaging flow cytometry preintervention/postintervention.
RESULTS
The INT exercise increased V˙O2peak (P = 0.04) compared with CONT training. Although training had no effect on platelet or leukocyte EV, INT decreased Annexin V- endothelial EV CD105 compared with CONT (P = 0.04). However, after accounting for dietary sugar intake, the intensity effect was lost (P = 0.18). Increased ad libitum dietary sugar intake after training was linked to elevated AV+ CD105 (r = 0.49, P = 0.06) and AV- CD45 (r = 0.59, P = 0.01). Nonetheless, increased V˙O2peak correlated with decreased AV+ CD105 (r = -0.60, P = 0.01).
CONCLUSIONS
Interval exercise training decreases endothelial-derived EV in adults with prediabetes. Although increased sugar consumption may alter EV after a short-term exercise intervention, fitness modifies EV count.
背景
细胞外囊泡（EV）被认为介导 2 型糖尿病和心血管疾病（CVD）的风险和发展。身体活动和均衡饮食可降低疾病风险，但尚无研究检验过以下假设，即短期间歇（INT）训练与成年人的糖尿病前期相比，会降低 EV 水平。CONT 运动。
方法
对 18 名肥胖成年人（年龄，63.8 ± 1.5 岁；体重指数，31.0 ± 1.3 kg·m）进行了糖尿病前期筛查，使用美国糖尿病协会标准（75 g 口服葡萄糖耐量试验）。受试者被随机分配到 INT（n = 10，分别以 90%和 50% HRpeak 的 3 分钟间隔交替）或 CONT（n = 8，70% HRpeak）训练，共 13 天，每天 60 分钟，共 12 次监督训练。心肺功能（V˙O2peak）、体重（kg）以及随意饮食摄入情况均进行了评估，在最后一次运动后 24 小时，通过平板压力测量法计算出总 AUC 以计算动脉僵硬（增强指数）。在干预前/干预后，通过成像流式细胞术分析总 EV、血小板 EV（CD31/CD41）、内皮 EV（CD105；CD31/CD41）、血小板内皮细胞黏附分子（PECAM）（CD31）和白细胞 EV（CD45；CD45/CD41）。
结果
与 CONT 训练相比，INT 运动增加了 V˙O2peak（P = 0.04）。尽管训练对血小板或白细胞 EV 没有影响，但与 CONT 相比，INT 降低了 Annexin V-内皮 EV CD105（P = 0.04）。然而，考虑到饮食中的糖摄入量后，强度效应消失（P = 0.18）。训练后随意饮食中糖摄入量的增加与 AV+ CD105 的升高相关（r = 0.49，P = 0.06）和 AV- CD45（r = 0.59，P = 0.01）。尽管如此，V˙O2peak 的增加与 AV+ CD105 的减少相关（r = -0.60，P = 0.01）。
结论
间歇运动训练可降低糖尿病前期成年人的内皮衍生 EV。尽管短期运动干预后糖的消耗增加可能会改变 EV，但身体活动会改变 EV 计数。
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