使用不同组合的 . 的PI-2a菌毛亚基抗原免疫小鼠。 （原文中“of”后面内容缺失）
Immunizing mice using different combination antigens of the PI-2a fimbria subunit of .
作者信息
Wang J L, Bu R E, Wu J H, Xi L G W, Chen J L, Sun L J, Wang H
机构信息
Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, 256600, China.
Inner Mongolia Autonomous Region Engineering Technology Research Center of Prevention and Control the Pathogenic Bacteria in Milk, Tongliao 028043, China.
出版信息
Iran J Vet Res. 2019 Summer;20(3):199-203.
BACKGROUND
is the main causal pathogen of bovine mastitis (BM), causing considerable economic loss to the dairy industry worldwide. Vaccines against play an important role in preventing disease.
AIMS
The aim of this study was to evaluate the immunoprotection of pilus island fusion proteins, ancillary protein 1-ancillary protein 2 (AP1-AP2), ancillary protein 1-bone protein (AP1-BP), bone protein-ancillary protein 2 (BP-AP2), and ancillary protein 1-bone protein-ancillary protein 2 (AP1-BP-AP2) in Balb/c mice.
METHODS
Four kinds of fusion antigens and the same volume of Freund's complete adjuvant were mixed vigorously to prepare fusion antigen immuno-samples. The mice were immunized 4 times (on the 0th, 7th, 14th, and 28th days) with these samples with an immunizing dose of 50 g/mouse. After the 4th immunization, serology tests were used to evaluate the antibody. The antibody titre produced by AP1-BP-AP2 fusion antigen was the highest, at up to 1:25600. The mice were then injected with 0.5 ml of 2 × 10 CFU/ml clinically isolated at day 50 and observed daily for the following 7 days.
RESULTS
Statistical analyses showed that these 4 kinds of fusion antigens had good protective immunity. Among them, AP1-BP-AP2 fusion antigen had the best protective immunity in Balb/c mice, with an immune protection index (PI) of 80%. This research provides a reliable theoretical basis for screening candidate antigens of the subunit vaccine and detecting antigen preparations of .
背景
是牛乳腺炎（BM）的主要致病病原体，给全球乳制品行业造成了巨大的经济损失。针对的疫苗在预防疾病方面发挥着重要作用。
目的
本研究旨在评估菌毛岛融合蛋白、辅助蛋白1 - 辅助蛋白2（AP1 - AP2）、辅助蛋白1 - 骨蛋白（AP1 - BP）、骨蛋白 - 辅助蛋白2（BP - AP2）和辅助蛋白1 - 骨蛋白 - 辅助蛋白2（AP1 - BP - AP2）在Balb/c小鼠中的免疫保护作用。
方法
将四种融合抗原与等量的弗氏完全佐剂充分混合，制备融合抗原免疫样本。小鼠在第0、7、14和28天用这些样本进行4次免疫，免疫剂量为50μg/只小鼠。第4次免疫后，采用血清学检测评估抗体。AP1 - BP - AP2融合抗原产生的抗体效价最高，可达1:25600。然后在第50天给小鼠注射0.5ml 2×10⁸CFU/ml临床分离株，并在接下来的7天每天观察。
结果
统计分析表明，这四种融合抗原具有良好的保护性免疫。其中，AP1 - BP - AP2融合抗原在Balb/c小鼠中具有最佳的保护性免疫，免疫保护指数（PI）为80%。本研究为筛选亚单位疫苗的候选抗原和检测的抗原制剂提供了可靠的理论依据。
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