猪肺泡巨噬细胞中感染 PRRSV 的长非编码 RNA 和 mRNA 谱的全基因组分析。
Genome-wide analysis of long noncoding RNA and mRNA profiles in PRRSV-infected porcine alveolar macrophages.
机构信息
Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan 430064, China.
Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan 430064, China.
出版信息
Genomics. 2020 Mar;112(2):1879-1888. doi: 10.1016/j.ygeno.2019.10.024. Epub 2019 Nov 1.
Porcine reproductive and respiratory syndrome (PRRS), which is caused by PRRS virus (PRRSV), is one of the most globally devastating swine diseases. It is essential to develop new strategy to control PRRS via an understanding of mechanisms that PRRSV utilizes to interfere with the host's innate immunity. In this study, we deeply sequenced and analyzed long noncoding RNA (lncRNA) and mRNA expression profiles of the porcine alveolar macrophages (PAMs) after PRRSV infection. 126 lncRNAs and 753 mRNAs were differentially expressed between PRRSV-infected and control PAMs. The co-expressed genes of down-regulated lncRNAs were significantly enriched within NF-kappa B and toll-like receptor signaling pathways. Co-expression network analysis indicated that part of the dysregulated lncRNAs associated with the interferon-induced genes. These dysregulated lncRNAs may play an important role in the host's innate immune responses to PRRSV infection. However, further research is required to characterize the function of these lncRNAs.
猪繁殖与呼吸综合征（PRRS）由猪繁殖与呼吸综合征病毒（PRRSV）引起，是全球危害最严重的猪病之一。通过了解 PRRSV 利用何种机制来干扰宿主固有免疫，从而开发新的策略来控制 PRRS 至关重要。在这项研究中，我们对感染 PRRSV 后的猪肺泡巨噬细胞（PAMs）中的长非编码 RNA（lncRNA）和 mRNA 表达谱进行了深度测序和分析。PRRSV 感染的 PAMs 与对照 PAMs 之间有 126 个 lncRNA 和 753 个 mRNA 表达存在差异。下调的 lncRNA 的共表达基因在 NF-kappa B 和 Toll 样受体信号通路中显著富集。共表达网络分析表明，部分失调的 lncRNA 与干扰素诱导基因有关。这些失调的 lncRNA 可能在宿主对 PRRSV 感染的固有免疫反应中发挥重要作用。然而，需要进一步研究来表征这些 lncRNA 的功能。
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