盘尾丝虫免疫显性抗原的分子克隆
Molecular cloning of an immunodominant antigen of Onchocerca volvulus.
作者信息
Lucius R, Erondu N, Kern A, Donelson J E
机构信息
Institute fur Tropenhygiene, Heidelberg, Federal Republic of Germany.
出版信息
J Exp Med. 1988 Sep 1;168(3):1199-204. doi: 10.1084/jem.168.3.1199.
Abstract
We have cloned and characterized the gene for an immunodominant antigen of O. volvulus that is recognized by the sera of 96% of patients with onchocerciasis. Its 1.2-kb mRNA constitutes 0.3% of adult worm poly(A)+ RNA and its cDNA sequence reveals that it is not a highly conserved structural protein such as actin or tubulin. Similar but not identical genes occur in the genomes of related filarie, Brugia malayi and Dirofilaria immitis. The recombinant antigen has both immunodiagnostic and immunoprophylactic significance.
摘要
我们已经克隆并鉴定了盘尾丝虫一种免疫显性抗原的基因，96%的盘尾丝虫病患者血清可识别该抗原。其1.2kb的mRNA占成虫多聚腺苷酸加尾RNA的0.3%，其cDNA序列显示它不是肌动蛋白或微管蛋白等高度保守的结构蛋白。在相关丝虫马来布鲁线虫和犬恶丝虫的基因组中存在相似但不相同的基因。该重组抗原有免疫诊断和免疫预防意义。
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