School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
J Biotechnol. 2020 Jan 10;307:125-130. doi: 10.1016/j.jbiotec.2019.11.007. Epub 2019 Nov 11.
Catalytic transformation of biomass-derived furans into value-added chemicals and biofuels has received considerable interest recently. In this work, aldehyde dehydrogenases (ALDHs) were identified from Comamonas testosteroni SC1588 for the oxidation of bio-based furans into furan carboxylic acids. Of the whole-cell biocatalysts constructed, Escherichia coli expressing a vanillin dehydrogenase (E. coli_CtVDH1) proved to be the best for the oxidation of 5-hydroxymethylfurfural (HMF). 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) was obtained in the yield of approximately 92 % within 12 h using this recombinant strain when the HMF concentration was up to 200 mM. In a fed-batch process, 292 mM of HMFCA was produced within 20.5 h, thereby providing a productivity as high as 2.0 g/L h. Other furan carboxylic acids were synthesized in the yields of 83-95%. Besides, the partially purified HMF was smoothly converted into HMFCA by this recombinant strain, with a 90% yield.
最近，人们对将生物质衍生的呋喃转化为有价值的化学品和生物燃料的催化转化产生了浓厚的兴趣。在这项工作中，从 Comamonas testosteroni SC1588 中鉴定出醛脱氢酶（ALDHs），用于将生物基呋喃氧化为呋喃羧酸。在所构建的全细胞生物催化剂中，表达香草醛脱氢酶（E. coli_CtVDH1）的大肠杆菌被证明是氧化 5-羟甲基糠醛（HMF）的最佳选择。当 HMF 浓度高达 200 mM 时，使用该重组菌在 12 h 内可获得约 92%的 5-羟甲基-2-呋喃羧酸（HMFCA）。在分批进料过程中，在 20.5 h 内生产了 292 mM 的 HMFCA，从而提供了高达 2.0 g/L/h 的生产率。其他呋喃羧酸的产率为 83-95%。此外，该重组菌可将部分纯化的 HMF 顺利转化为 HMFCA，收率为 90%。
