School of Arts, Sciences and Humanities, Textile and Fashion Course, University of São Paulo, Av. Arlindo Bétio, 1000, Parque Ecológico do Tietê, Ermelino Matarazzo, CEP: 03828-080, São Paulo, SP, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Vila Universitária, 17012901, Bauru, SP, Brazil.
Enzyme Microb Technol. 2020 Jan;132:109384. doi: 10.1016/j.enzmictec.2019.109384. Epub 2019 Aug 9.
Cellulose triacetate (CTA) synthesized by cellulose extracted from sugarcane bagasse, and commercial cellulose acetate (CA) were used to produce nanofiber membranes contained bromelain by electrospinning technique. About 1.3 g of cellulose acetate per gram of bagasse were obtained, and both CTA and CA was characterized by analysis of Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). The nanofiber membranes were produced by electrospinning process testing the following conditions: voltage 25 kV, flow rate 4 mL/h and distance 10 cm, using acetone/ dimethylformamide (DMF) (85:15 m/ m) to 15% cellulose triacetate (70% CA + 30% CTA) or CA solutions. Scanning Electron Microscopy (SEM) was used to nanofiber membranes characterization. Bromelain was immobilized on the nanofiber membranes by crosslinking with glutaraldehyde and directly in the electrospinning step, the highest activity recovery was about 675% and in vitro controlled release tests were performed to semi-quantitatively evaluate the release of the enzyme bromelain thus demonstrating complete release process in 3 days.
采用甘蔗渣提取的纤维素合成三醋酸纤维素（CTA）和商用醋酸纤维素（CA），通过静电纺丝技术制备含菠萝蛋白酶的纳米纤维膜。每克蔗渣可获得约 1.3g 的醋酸纤维素，通过傅里叶变换红外光谱（FTIR）和差示扫描量热法（DSC）对 CTA 和 CA 进行了分析。通过静电纺丝工艺制备纳米纤维膜，测试了以下条件：电压 25kV、流速 4mL/h 和距离 10cm，使用丙酮/二甲基甲酰胺（DMF）（85:15m/m）溶解 15%三醋酸纤维素（70%CA+30%CTA）或 CA 溶液。采用扫描电子显微镜（SEM）对纳米纤维膜进行了表征。通过戊二醛交联和直接在静电纺丝步骤中固定菠萝蛋白酶，固定化酶的最高酶活回收率约为 675%，并进行了体外控释试验，对酶菠萝蛋白酶的释放进行了半定量评估，结果表明在 3 天内实现了完全释放过程。
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