College of Forestry, Shandong Agricultural University, No. 61, Daizong Road, Tai' an 271018, China.
Shandong Provincial Research Center of Demonstration Engineering Technology for Urban and Rural Landscape, Tai' an 271018, China.
Int J Mol Sci. 2019 Nov 26;20(23):5953. doi: 10.3390/ijms20235953.
Many members of the WRKY family regulate plant growth and development. Recent studies have shown that members of the WRKY family, specifically WRKY13, play various roles in the regulation of plant stress resistance. To study the function of WRKY family members in peony, the gene (KY271095) was cloned from peony leaves. Sequence analysis and subcellular localization results revealed that has no introns, belongs to the type IIc subgroup of the WRKY family, and functions in the nucleus. The expression pattern of was analysed via real-time quantitative RT-PCR (qRT-PCR), which showed that the expression of was induced by four types of abiotic stress, low-temperature, high-temperature, waterlogging and salt stress, and was positively upregulated in response to these stresses. In addition, the expression of tended to first decrease and then increase after infection with . Virus-induced gene silencing (VIGS) technology was used to explore the function of in the resistance of to fungal infection further, and the results showed that -silenced plants displayed increased sensitivity to The infection was more severe and the disease index (DI) significantly greater in the -silenced plants than in the control plants, and the expression of pathogenesis-related (PR) genes was also significantly altered in the -silenced plants compared with the control plants. The contents of the endogenous hormones jasmonic acid (JA) and salicylic acid (SA) were measured, and the results showed that the JA content increased gradually after infection with and that JA may play an active role in the resistance of to pathogen infection, while the SA content decreased after silencing. The contents of the two hormones decreased overall, suggesting that they are related to the transcription of and that may be involved in the disease-resistance pathway mediated by JA and SA. In summary, the results of our study showed that expression was induced by stress and had a positive effect on the resistance of to fungal infection.
许多 WRKY 家族成员参与植物生长发育的调控。近期研究表明，WRKY 家族成员（尤其是 WRKY13）在植物抗逆性调节中发挥多种作用。为研究 WRKY 家族成员在牡丹中的功能，本研究从牡丹叶片克隆得到一个基因（KY271095）。序列分析和亚细胞定位结果表明，该基因无内含子，属于 WRKY 家族的 IIc 亚组，定位于细胞核。实时定量 RT-PCR（qRT-PCR）分析表明， 受低温、高温、淹水和盐胁迫诱导，且对这些胁迫呈正上调表达。此外， 受 侵染后表达量先下降后上升。进一步利用病毒诱导的基因沉默（VIGS）技术探究 对真菌侵染的抗性功能，结果表明， -沉默植株对真菌侵染的敏感性增加，表现出更严重的症状，病指（DI）显著高于对照植株，且病程相关（PR）基因的表达也显著改变。测定了内源激素茉莉酸（JA）和水杨酸（SA）的含量，结果表明， 侵染后 JA 含量逐渐增加，JA 可能在牡丹抵抗病原菌侵染中发挥积极作用，而 SA 含量在 沉默后下降。两种激素含量总体下降，表明它们与 转录有关， 可能参与 JA 和 SA 介导的抗病途径。综上所述，本研究结果表明， 表达受胁迫诱导，对牡丹抵抗真菌侵染具有正向作用。
