喉鳞癌患者 rs12778366、rs2981582、rs744166 和 rs1800625 单核苷酸基因多态性的测定。
Determination of rs12778366, rs2981582, rs744166, and rs1800625 Single Gene Polymorphisms in Patients with Laryngeal Squamous Cell Carcinoma.
机构信息
Department of Otolaryngology, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania.
出版信息
Dis Markers. 2019 Nov 12;2019:3907232. doi: 10.1155/2019/3907232. eCollection 2019.
PURPOSE
To determine the frequency of the genotype of signal transducer and activator of transcription protein 3 (STAT3) rs744166, sirtuin (SIRT1) rs12778366, fibroblast growth factor (FGFR2) rs2981582, and advanced glycosylation end product-specific receptor (RAGE) rs1800625 gene polymorphisms in patients with laryngeal squamous cell carcinoma (LSCC).
METHODS
A total of 944 subjects were evaluated, which includes 144 patients with LSCC and 800 healthy controls. The genotyping of rs744166, rs12778366, rs2981582, and rs1800625 was carried out using the RT-PCR.
RESULTS
The analysis of rs744166, rs12778366, and rs2981582 gene polymorphisms did not reveal any differences in genotype distribution between the patients with LSCC and the control subjects. However, statistical analysis revealed that genotypes (AA, AG, and GG) of rs1800625 in gene were distributed statistically significantly differently between patients and controls (61.1%, 30.6%, and 23.6% vs. 72.5%, 25.8%, and 1.8%, respectively; < 0.001). Additionally, statistical significance was observed in allele distribution between these two groups, i.e., allele G at rs1800625 was more frequently observed in the patient group than in controls (23.6% vs. 14.6%; < 0.001).
CONCLUSION
rs1800625 gene polymorphism may play a significant role in laryngeal squamous cell carcinoma development.
目的
检测信号转导和转录激活因子 3（STAT3）rs744166、沉默调节蛋白 1（SIRT1）rs12778366、成纤维细胞生长因子（FGFR2）rs2981582 和晚期糖基化终产物特异性受体（RAGE）rs1800625 基因多态性在喉鳞状细胞癌（LSCC）患者中的发生频率。
方法
共纳入 944 例研究对象，包括 144 例 LSCC 患者和 800 例健康对照者。采用 RT-PCR 技术对 rs744166、rs12778366、rs2981582 和 rs1800625 基因多态性进行检测。
结果
rs744166、rs12778366 和 rs2981582 基因多态性分析未发现 LSCC 患者与对照组在基因型分布上存在差异。然而，统计学分析显示 rs1800625 基因的基因型（AA、AG 和 GG）在患者与对照组之间存在统计学差异（61.1%、30.6%和 23.6%与 72.5%、25.8%和 1.8%；<0.001）。此外，两组间等位基因分布也存在统计学差异，即 rs1800625 中的等位基因 G 在患者组中更为常见（23.6%比 14.6%；<0.001）。
结论
rs1800625 基因多态性可能在喉鳞状细胞癌的发生发展中发挥重要作用。
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