转录因子早期生长反应-1在唾液腺多形性腺瘤中发挥致癌作用。

Transcription factor early growth response-1 plays an oncogenic role in salivary gland pleomorphic adenoma.

机构信息

Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, No. 639, Zhizaoju Road, Huangpu District, Shanghai, 200011, People's Republic of China.

出版信息

Biotechnol Lett. 2020 Feb;42(2):197-207. doi: 10.1007/s10529-019-02776-1. Epub 2019 Nov 30.

DOI:10.1007/s10529-019-02776-1

PMID:31786685

Abstract

OBJECTIVES

Although abnormal expression of early growth response-1 (Egr1) has been revealed in various human solid tumors, the functions and potential mechanisms of Egr1 in the progression of salivary gland pleomorphic adenoma (SGPA) are not entirely understood.

RESULTS

An elevated expression of Egr1 was observed both in the human salivary gland pleomorphic adenoma tissues and tumor-initiating cell (TIC) cells, when compared with control group. By loss-of-function assay, the proliferation and invasion capacities of TICs were inhibited, while the cell apoptosis was promoted, which were further evidenced by the protein expression analysis of several key apoptosis-related regulators. Furthermore, TICs with Mithramycin A (an Egr1 inhibitor) treatment achieved the same effects of endogenous Egr1 knockdown.

CONCLUSIONS

All these data collectively suggest that Egr1 act as an oncogenic factor in salivary gland pleomorphic adenoma, which may be a potential target for the treatment of SGPA.

摘要

目的

尽管早期生长反应因子-1（Egr1）的异常表达已在各种人类实体瘤中被揭示，但 Egr1 在唾液腺多形性腺瘤（SGPA）进展中的功能和潜在机制尚不完全清楚。

结果

与对照组相比，人唾液腺多形性腺瘤组织和肿瘤起始细胞（TIC）中均观察到 Egr1 的表达升高。通过功能丧失试验，TIC 的增殖和侵袭能力受到抑制，而细胞凋亡受到促进，这进一步通过几种关键凋亡相关调节剂的蛋白表达分析得到证实。此外，用米托蒽醌 A（Egr1 抑制剂）处理 TIC 可获得与内源性 Egr1 敲低相同的效果。