二异丙基氨基磺酸钠（DIDS）对牛红细胞渗透特性的影响。

Effect of DIDS on osmotic properties of bovine erythrocytes.

作者信息

Mosior M, Białas W A, Gomułkiewicz J

机构信息

Institute of Physics, Technical University of Wrocław, Poland.

出版信息

Biochim Biophys Acta. 1988 Nov 3;945(1):51-5. doi: 10.1016/0005-2736(88)90361-6.

DOI:10.1016/0005-2736(88)90361-6

PMID:3179310

Abstract

The effect of DIDS on osmotic properties of bovine erythrocytes was studied. The isoosmotic volume, the amount of intracellular solutes, and the osmotically non-active volume were not influenced by DIDS. An increase of osmotic fragility of erythrocytes upon DIDS treatment was evident and identical both in NaCl and in NaCl + KCl hypotonic solutions. These results suggest that the critical cell volume decreases. The link between the effect of DIDS on the membrane skeleton extractability and on the osmotic fragility was postulated.

摘要

研究了二异丙基氟磷酸（DIDS）对牛红细胞渗透特性的影响。等渗体积、细胞内溶质含量和渗透非活性体积不受DIDS影响。在DIDS处理后，红细胞的渗透脆性增加，在氯化钠和氯化钠+氯化钾低渗溶液中均很明显且相同。这些结果表明临界细胞体积减小。推测了DIDS对膜骨架可提取性的影响与对渗透脆性的影响之间的联系。