一种用于原发性和转移性结直肠癌的新型活体 Cerenkov 光图像引导手术。

A novel in vivo Cerenkov luminescence image-guided surgery on primary and metastatic colorectal cancer.

机构信息

Engineering Research Center of Molecular and Neuro Imaging of Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, China.

CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China.

出版信息

J Biophotonics. 2020 Mar;13(3):e201960152. doi: 10.1002/jbio.201960152. Epub 2019 Dec 16.

DOI:10.1002/jbio.201960152

PMID:31800171

Abstract

Intraoperative Cerenkov luminescence imaging (CLI) can effectively improve the performance of tumor surgery. Nevertheless, the existing approaches are still unsatisfying to the clinical demands of open surgery. This study develops a novel intraoperative in vivo CLI approach to investigate the potential and value of Cerenkov luminescence (CL) image-guided surgery. A system characterized with high sensitivity (19.61 kBq mL F-FDG) and desirable spatial resolution (88.34 μm) is developed. CL image-guided surgery is performed on colorectal cancer (CRC) models of mice and swine. Tumor surgery is guided by the static CL images, and the resection quality is evaluated quantitatively and contrasted with other imaging modalities exemplified by bioluminescence imaging (BLI). The in vivo results demonstrated the effectiveness of the proposed intraoperative CLI approach for removing primary and metastatic CRC. Safety of performing in vivo CL image-guided surgery is verified as well through radiation measurements of related staffs. Overall, the developed intraoperative in vivo CLI approach can efficiently improve the cancer treatment.

摘要

术中切伦科夫发光成像（CLI）可有效提高肿瘤手术的性能。然而，现有的方法仍不能满足开放式手术的临床需求。本研究开发了一种新的术中体内 CLI 方法，以研究切伦科夫发光（CL）图像引导手术的潜力和价值。开发了一种具有高灵敏度（19.61 kBq mL F-FDG）和理想空间分辨率（88.34 μm）的系统。对小鼠和猪的结直肠癌（CRC）模型进行了 CL 图像引导手术。通过静态 CL 图像引导肿瘤手术，并定量评估切除质量，并与生物发光成像（BLI）等其他成像方式进行对比。体内结果表明，所提出的术中 CLI 方法对于切除原发性和转移性 CRC 是有效的。通过对相关工作人员的辐射测量，验证了进行体内 CL 图像引导手术的安全性。总的来说，所开发的术中体内 CLI 方法可以有效地提高癌症治疗效果。