用于检测人血清样品中毫摩尔葡萄糖的酶法及其验证。

Enzymatic method and its validation for the micromolar assay of glucose in human serum samples.

机构信息

Department of Chemistry, JSS Academy of Technical Education, (Affiliated to Visveswaraya Technological University, Belagavi), Dr. Vishnuvardhan Road, Bengaluru, 560060, Karnataka, India.

出版信息

Anal Biochem. 2020 Feb 1;590:113536. doi: 10.1016/j.ab.2019.113536. Epub 2019 Dec 7.

DOI:10.1016/j.ab.2019.113536

PMID:31821804

Abstract

This work depicts the novel chromogenic system for the assay of glucose in blood samples connected with the chelate formation of sulfosalicylic acid (SSA) with iron (Fe(III)) using glucose oxidase (GOD) presented. The purple-colored Fe-SSA chelate compound formed, maintain a strong absorption at 500 nm in the acidic buffer of pH 3.8. The Beer's law limit for the assay of glucose by rate, and a one-time method is in the range of 46-1295 μmol/L and 9-1110 μmol/L sequentially. Inter and Intraday precision fluctuated amid 0.98-1.4% (n = 10), and 1.33-2.89% (n = 15) sequentially. The recovery of glucose varied from 96.6 to 102%, registering trifling interference by common interferants in blood samples. The accuracy of the outcome, LOD, and LOQ of glucose were within 90-102%, 2.376, and 7.923 μmol/L individually. The introduced method has a coefficient of correlation 0.999 with the standard kit system, and easy to ascertain serum glucose with excellent recovery and insignificant interruption. Nevertheless, the system can be recognized for selection by the biochemical laboratories.

摘要

这项工作描绘了一种用于检测血液样本中葡萄糖的新型显色体系，该体系涉及到使用葡萄糖氧化酶（GOD）形成磺基水杨酸（SSA）与铁（Fe(III)）的螯合物。形成的紫色 Fe-SSA 螯合物在 pH 3.8 的酸性缓冲液中保持在 500nm 处的强吸收。通过速率法测定葡萄糖的比尔定律限和一次性方法分别在 46-1295μmol/L 和 9-1110μmol/L 范围内。日内和日间精密度分别在 0.98-1.4%（n=10）和 1.33-2.89%（n=15）之间波动。葡萄糖的回收率在 96.6%至 102%之间变化，在血液样本中仅记录到微量的常见干扰物的干扰。该方法的准确性、检测限和定量限分别为 90-102%、2.376 和 7.923μmol/L。该方法与标准试剂盒系统具有 0.999 的相关系数，可用于确定血清葡萄糖，具有良好的回收率和可忽略的干扰。然而，该系统可以被生化实验室认可选择。