1期和2期牙周炎患者唾液酸和白细胞介素10水平的评估。
Estimation of Sialic Acid and IL10 Levels in Stage 1 and 2 Periodontitis Patients.
作者信息
Varma Sudhir Rama, Thomas Biju, Ramesh Amitha, Kumari Suchetha, Muhammad Al Saadi Zainab Abdullah, Mahmood Rabia Asif
机构信息
Department of Periodontics, University of Science and Technology of Fujairah, Fujairah, UAE.
Department of Periodontics, AB Shetty Memorial Institute of Dental Sciences, Mangaluru, Karnataka, India.
出版信息
Int J Dent. 2019 Nov 29;2019:2917124. doi: 10.1155/2019/2917124. eCollection 2019.
OBJECTIVE
The role of biomarkers in staging and grading periodontal disease has become detrimental in relation to the overall treatment plan. This study aimed at evaluating and comparing the role of sialic acid and IL10 in the early and moderate stages of periodontitis.
MATERIALS AND METHODS
Patients were selected according to the assessment of pocket depth and radiographic bone loss. Bone loss was calculated as <15% for stage 1 and 15-33% for stage 2. Salivary samples were collected using spit technique 2 hrs post consumption of food. The unstimulated saliva was collected in a sterile graduated container every minute for 5-8 minutes. IL10 estimation was done using ELISA, and sialic acid estimation was done using the diphenylamine method. The variables for the three groups were assessed using ANOVA, and intragroup comparisons for quantitative data were evaluated using the post hoc Bonferroni test ( < 0.05).
RESULTS
On comparing sialic acid levels among the three groups, stage 2 showed the highest mean (8.61) compared with the other two groups and was highly significant ( < 0.001). On the contrary, IL10 when compared to stage 1 and 2 periodontitis revealed insignificant change.
CONCLUSION
The value of IL10 was higher as patients progressed from health to periodontitis.
目的
生物标志物在牙周疾病分期和分级中的作用对于整体治疗计划已变得至关重要。本研究旨在评估和比较唾液酸和白细胞介素10（IL10）在牙周炎早期和中期阶段的作用。
材料与方法
根据牙周袋深度和影像学骨丧失评估选择患者。骨丧失计算为：1期小于15%，2期为15%至33%。进食2小时后采用吐唾技术收集唾液样本。每分钟在无菌刻度容器中收集未刺激唾液，持续5至8分钟。使用酶联免疫吸附测定法（ELISA）进行IL10测定，使用二苯胺法进行唾液酸测定。使用方差分析（ANOVA）评估三组的变量，使用事后邦费罗尼检验（<0.05）评估定量数据的组内比较。
结果
比较三组之间的唾液酸水平，2期的平均水平最高（8.61），与其他两组相比差异高度显著（<0.001）。相反，与1期和2期牙周炎相比，IL10显示无显著变化。
结论
随着患者从健康发展到牙周炎，IL10的值更高。
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