下肢肌肉力量和 5-羟色胺受体基因多态性与纤维肌痛女性相关的因素。

Rehabilitation Sciences State University of Londrina and University Pitágoras Unopar (UEL/UNOPAR), Londrina, PR, Brazil.

Departament of Physical Education and Sport, Londrina State University (UEL), Londrina, Paraná, Brazil.

Adv Rheumatol. 2019 Dec 26;59(1):59. doi: 10.1186/s42358-019-0101-9.

BACKGROUND

Fibromyalgia (FM) is a chronic pain syndrome characterized by generalized skeletal muscle chronic pain. Its etiology is not well defined, because there are several factors that may trigger it such as physical and/or emotional stresses, or a genetic susceptibility, involving serotonergic, dopaminergic and catecholaminergic paths. The objective of this study was to investigate the association between the strength of the lower limb, genetic polymorphism of the serotonin receptor gene HTR in women with fibromyalgia.

METHODS

In this observational study of case-control type 48 women were evaluated who belonged to the group with FM (52 ± 12 years) and 100 women in the control group (58 ± 11 years). Socio demographic and anthropometric data were collected and peripheral blood samples for DNA extraction; genotypic analyzes were performed by means of PCR in real time by TaqMan® system. The lower limb muscle strength was assessed through the test of sitting down and standing up for 30 s. The chi-square test or Fischer Exact was used for possible associations among the variables; the t-test for independent samples was used to compare the averages among the groups; the value of significance adopted was 5%.

RESULTS

There was an association between the polymorphism of the HTR2A gene with FM, demonstrating that carriers of the genotype GG have 24.39 times more likely to develop the syndrome (IC95% 5.15-115.47; p = 0.01). It was observed an association between FM and the test to sit and stand up demonstrating that women with fibromyalgia have lower limb muscle strength (p = 0.01). The study showed that the white race has 3.84 times more likely to develop FM (p = 0.01).

CONCLUSION

The results of this study suggest that women of Caucasian ethnicity with GG genotype or G allele presented greater risk of developing fibromyalgia and that these patients have lower limb muscle strength compared to the control group.

背景

纤维肌痛（FM）是一种以全身骨骼肌慢性疼痛为特征的慢性疼痛综合征。其病因尚不清楚，因为有几个可能导致纤维肌痛的因素，如身体和/或情绪压力，或遗传易感性，涉及 5-羟色胺能、多巴胺能和儿茶酚胺能途径。本研究的目的是探讨纤维肌痛女性下肢力量与 5-羟色胺受体基因 HTR 遗传多态性的关系。

方法

本研究为病例对照观察性研究，共纳入 48 例纤维肌痛女性患者（52±12 岁）和 100 例对照组女性（58±11 岁）。收集社会人口学和人体测量学数据，并采集外周血样进行 DNA 提取；采用 TaqMan®系统实时 PCR 进行基因分型分析。通过 30 秒坐站测试评估下肢肌肉力量。采用卡方检验或 Fischer 确切概率法分析可能存在的变量间关联；采用独立样本 t 检验比较组间平均值；采用 5%作为显著性检验水准。

结果

HTR2A 基因多态性与 FM 存在关联，表明 GG 基因型携带者发生该综合征的风险增加 24.39 倍（95%CI95%为 5.15-115.47；p=0.01）。还观察到 FM 与坐站测试之间的关联，表明纤维肌痛女性下肢肌肉力量较弱（p=0.01）。研究表明，白人患 FM 的风险增加 3.84 倍（p=0.01）。