Promiscuity and sex ratio in the terrestrial isopod Armadillidium vulgare and consequences on genetic diversity.

In promiscuous mating systems, both males and females mate with several partners. While the benefits of multiple mating are well recognized for males, there are several non-mutually exclusive hypotheses to explain multiple mating for females. Promiscuity is widespread in terrestrial isopods. Here, we placed experimental populations of the terrestrial isopod Armadillidium vulgare under varying sex ratios to manipulate the number of available partners, and better characterise the mating system in this species by performing paternity tests using microsatellite markers. We observed that females usually mate multiply with up to 5 males in a single event of reproduction. A higher number of fathers in broods did not increase brood size nor heterozygosity, but increased allelic richness. Promiscuity seems to be asymmetric in this species, with females being less affected by changes in sex ratio than were males and would be an adaptive mating system maximising the offspring genetic diversity.