类风湿关节炎中 H19、GAS5 和 linc0597 表达降低及相关基因多态性的关联分析。
Decreased H19, GAS5, and linc0597 Expression and Association Analysis of Related Gene Polymorphisms in Rheumatoid Arthritis.
机构信息
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, China.
Anhui Province Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei 230032, China.
出版信息
Biomolecules. 2019 Dec 29;10(1):55. doi: 10.3390/biom10010055.
Long noncoding RNAs (lncRNAs) widely participate in human diseases by regulating gene transcription, modulating protein function, or acting as ceRNAs. Yet, their roles in rheumatoid arthritis (RA) remain obscure. In this study, the expression of three lncRNAs (H19, GAS5, and linc0597) in peripheral blood mononuclear cells (PBMCs) were detected in 77 RA patients and 78 controls using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The association of lncRNAs related gene polymorphisms with RA were evaluated in 828 RA patients and 780 controls using TaqMan single nucleotide polymorphism (SNP) genotyping assays. We observed that the expression levels of H19, GAS5 and linc0597 were down-regulated in PBMCs of RA patients, of which GAS5 level decreased in patients with hypocomplementemia, and negatively correlated with C-reactive protein (CRP) level in RA patients. Moreover, we highlighted two related potential functional SNPs, GAS5 rs6790 and linc0597 rs2680700 for associations with RA susceptibility. The precise roles of these lncRNAs in mechanism of RA remain to be further explored.
长链非编码 RNA（lncRNAs）通过调节基因转录、调节蛋白质功能或作为 ceRNA 广泛参与人类疾病。然而，它们在类风湿关节炎（RA）中的作用仍不清楚。在这项研究中，我们使用定量实时逆转录聚合酶链反应（qRT-PCR）检测了 77 例 RA 患者和 78 例对照者外周血单个核细胞（PBMC）中 3 种 lncRNA（H19、GAS5 和 linc0597）的表达。使用 TaqMan 单核苷酸多态性（SNP）基因分型检测评估了 828 例 RA 患者和 780 例对照者中与 lncRNA 相关基因多态性与 RA 的相关性。我们观察到 RA 患者 PBMC 中 H19、GAS5 和 linc0597 的表达水平下调，其中 GAS5 水平在低补体血症患者中降低，与 RA 患者的 C 反应蛋白（CRP）水平呈负相关。此外，我们强调了两个相关的潜在功能 SNP，GAS5 rs6790 和 linc0597 rs2680700，与 RA 易感性相关。这些 lncRNA 在 RA 发病机制中的精确作用仍有待进一步探索。
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