番茄红素、[6]-姜辣素和水飞蓟宾对 U-118MG 脑胶质瘤细胞体外模型细胞凋亡的影响。
The Influence of Lycopene, [6]-Gingerol, and Silymarin on the Apoptosis on U-118MG Glioblastoma Cells In Vitro Model.
机构信息
Centre for Innovative Research in Medical and Natural Sciences, Medical Faculty of University of Rzeszow, 1a Warzywna St., 35-310 Rzeszow, Poland.
Department of Human Immunology, Medical Faculty of University of Rzeszow, 1a Warzywna St., 35-310 Rzeszow, Poland.
出版信息
Nutrients. 2019 Dec 30;12(1):96. doi: 10.3390/nu12010096.
BACKGROUND
Lycopene, gingerol, and silymarin have a potential anticancer activity in many types of neoplasms. Healthy lifestyle and proper diet are associated with a reduced risk of cancer and other diseases. Increasingly, clinical research focuses on the mechanisms of action of natural compounds and their impact on the development of cancer. The aim of the present study was to determine the effect of lycopene, gingerol, and silymarin on apoptosis, mitochondrial potential and caspase-3/7 activity in the U118-MG cell line.
METHODS
Human glioblastoma cells were incubated with lycopene, [6]-gingerol, and silymarin for 24 and 48 h. Apoptosis was monitored using the Annexin V labelling, caspase-3/7 activity, and early hallmark of apoptosis were determined with mitochondrial membrane potential changes.
RESULTS
Our data showed a significant decrease in the viability glioblastoma cells U118-MG after 48 h treatment with lycopene, [6]-gingerol, and silymarin.
CONCLUSIONS
Our data could confirm the stimulative effects of used compounds on apoptosis and changes in mitochondrial potential in a dose-dependent manner.
背景
番茄红素、姜黄素和水飞蓟素在多种肿瘤中具有潜在的抗癌活性。健康的生活方式和适当的饮食与降低癌症和其他疾病的风险有关。越来越多的临床研究关注天然化合物的作用机制及其对癌症发展的影响。本研究的目的是确定番茄红素、[6]-姜黄素和水飞蓟素对 U118-MG 细胞系细胞凋亡、线粒体膜电位和 caspase-3/7 活性的影响。
方法
用人神经胶质瘤细胞系 U118-MG 与番茄红素、[6]-姜黄素和水飞蓟素孵育 24 和 48 小时。用 Annexin V 标记法监测细胞凋亡，用 caspase-3/7 活性和线粒体膜电位变化来确定早期细胞凋亡的标志。
结果
我们的数据显示，番茄红素、[6]-姜黄素和水飞蓟素处理 48 小时后，人神经胶质瘤细胞系 U118-MG 的活力显著下降。
结论
我们的数据可以证实所使用的化合物以剂量依赖的方式刺激细胞凋亡和线粒体膜电位变化。
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