刺猬信号通路（Hedgehog）和磷脂酰肌醇-3-激酶-蛋白激酶B（PI3K-AKT）信号通路相关成分的表达与肝细胞癌的侵袭和转移相关。
The expressions of Hedgehog and PI3K-AKT pathway components correlate with invasion and metastasis in hepatocellular carcinoma.
作者信息
Chen Bin, Hu Zeming, Li Bofei, Lin Xuan, Luo Zhijiang, Hu Zhiqiang
机构信息
Department of General Surgery, The First Affiliated Hospital of Gannan Medical University Ganzhou 341000, Jiangxi Province, China.
Postgraduate Student, Gannan Medical University Ganzhou 341000, Jiangxi Province, China.
出版信息
Int J Clin Exp Pathol. 2019 Jun 1;12(6):2381-2388. eCollection 2019.
OBJECTIVE
To investigate the expression and clinical significance of Shh, Gli1, FAK, p-FAK and p-AKT in HCC.
METHODS
Immunohistochemistry was used to measure Shh, Gli1, FAK, p-FAK, and p-AKT expressions in 50 cases of HCC and paracancerous tissues. The Shh, Gli1, and FAK mRNA levels were determined by qRT-PCR in 20 HCCs. The correlations between the expressions of these target genes and the clinicopathological factors were analyzed in HCC.
RESULTS
The immunohistochemical results showed that the expressions of Shh, Gli1, FAK, p-FAK, and p-AKT in 50 HCC tissues were significantly higher than those of the paracancerous tissues (P < 0.05). Shh and p-FAK expressions were associated with portal vein invasion, capsular integrity, and distant metastasis (P < 0.05). Gli1, FAK, and p-AKT expressions were closely related to tumor diameter, tumor differentiation, portal vein invasion, capsular integrity, TNM stage and distant metastasis (P < 0.05). Shh was related to Gli1 and p-FAK (r = 0.67, 0.30; P = 0.00, 0.03), Gli1 was positively related to p-FAK and p-AKT (r = 0.52, 0.49; P = 0.00, 0.00), and there was a positive correlation between p-FAK and p-AKT (r = 0.36, P = 0.00). Furthermore, the Shh, Gli1, and FAK mRNA levels in the HCC tissues were significantly higher than those in the paracancerous tissues (P < 0.0001), and the high TNM stages (III and IV) or distant metastasis were significantly higher than those in the low TNM stages (I and II) (P < 0.05) or without distant metastasis (P < 0.05).
CONCLUSION
In HCC, the Hh and PI3K-AKT signaling pathways are both abnormally activated, and Shh, Gli1, FAK, p-FAK and p-AKT can serve as indicators to predict the prognosis of liver cancer.
目的
探讨Shh、Gli1、FAK、p-FAK和p-AKT在肝癌中的表达及临床意义。
方法
采用免疫组织化学法检测50例肝癌及癌旁组织中Shh、Gli1、FAK、p-FAK和p-AKT的表达。采用qRT-PCR法检测20例肝癌组织中Shh、Gli1和FAK的mRNA水平。分析这些靶基因表达与肝癌临床病理因素之间的相关性。
结果
免疫组织化学结果显示，50例肝癌组织中Shh、Gli1、FAK、p-FAK和p-AKT的表达均显著高于癌旁组织（P<0.05）。Shh和p-FAK的表达与门静脉侵犯、包膜完整性及远处转移有关（P<0.05）。Gli1、FAK和p-AKT的表达与肿瘤直径、肿瘤分化、门静脉侵犯、包膜完整性、TNM分期及远处转移密切相关（P<0.05）。Shh与Gli1及p-FAK相关（r=0.67，0.30；P=0.00，0.03），Gli1与p-FAK及p-AKT呈正相关（r=0.52，0.49；P=0.00，0.00），p-FAK与p-AKT呈正相关（r=0.36，P=0.
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