痕量水活性可以通过促进脂肪酶 RM IM 催化的酯交换反应中的酰基迁移来提高 1,3-油酸-2-中链富三酰基甘油的形成。
Trace water activity could improve the formation of 1,3-oleic-2-medium chain-rich triacylglycerols by promoting acyl migration in the lipase RM IM catalyzed interesterification.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; School of Public Health, Nanchang University, 461 Ba Yi Avenue, Nanchang, Jiangxi 330006, China.
出版信息
Food Chem. 2020 May 30;313:126130. doi: 10.1016/j.foodchem.2019.126130. Epub 2019 Dec 26.
New structured lipids with 1,3-oleic-2-medium chain (OMO) triacylglycerols were synthesized by promoting acyl migration in Lipozyme RM IM catalyzed interesterification between coconut oil (CO) and high oleic rapeseed oil (HORO). Results from an orthogonal design L(5) showed that the maximal yield of OMO-structured triacylglycerols was 45.65% under the following conditions: the molar ratio of CO to HORO, 50:50; enzyme dosage, 12 wt%; reaction temperature, 60 °C; reaction time, 2 h; water activity, 0.07. Low water activity showed a high rate of acyl migration (10.86% vs 5.07% no water system), which promoted OMO synthesis due to medium-chain fatty acid migration to the sn-2 position. In a low water content (5%) system of the molecular dynamics simulation, water molecules stabilized the whole structure of RM IM through hydrogen bonding, which helped fix lipase-catalyzed active sites, making substrates more easily inserted into active sites, resulting in increased enzyme activity.
新型 1,3-油酸-2-中链（OMO）三酰基甘油结构脂是通过在脂肪酶 RM IM 催化的椰子油（CO）和高油酸油菜籽油（HORO）的酯交换反应中促进酰基迁移合成的。正交设计 L(5)的结果表明，在以下条件下，OMO 结构三酰基甘油的最大产率为 45.65%：CO 与 HORO 的摩尔比为 50:50；酶用量为 12 wt%；反应温度为 60°C；反应时间为 2 h；水活度为 0.07。低水活度显示出较高的酰基迁移率（10.86%对无水系的 5.07%），这促进了 OMO 的合成，因为中链脂肪酸迁移到 sn-2 位置。在水含量为 5%的分子动力学模拟的低水系统中，水分子通过氢键稳定了 RM IM 的整个结构，这有助于固定脂肪酶催化的活性部位，使底物更容易插入活性部位，从而提高酶活性。
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