Andrzejczak-Grządko Sylwia, Dolińska Izabela
Katedra Mikrobiologii i Genetyki, Wydział Nauk Biologicznych, Uniwersytet Zielonogórski.
Zakład Diagnostyki Laboratoryjnej, Szpital Uniwersytecki im. K. Marcinkowskiego w Zielonej Górze.
Postepy Biochem. 2019 Oct 23;65(4):299-305. doi: 10.18388/pb.2019_273.
Urogenital mycoplasmas belonging to Mycoplasma and Ureaplasma genera, are both commensal and pathogenic microorganisms. The gold standard for their detection in clinical samples is culture and identification based on biochemical tests. However, this method is time-consuming and not very specific. Detection of mycoplasmas using PCR allows for assigning not only to the genus, but also to the species. In this study, 100 clinical samples were analyzed to identify M. hominis, M. genitalium, U. parvum and U. urealyticum, using both biochemical and molecular methods. The presented results confirm the low specificity of biochemical tests and the higher detection efficiency of individual species using molecular methods.
属于支原体属和脲原体属的泌尿生殖支原体既是共生微生物,也是致病微生物。在临床样本中检测它们的金标准是基于生化试验的培养和鉴定。然而,这种方法耗时且特异性不强。使用聚合酶链反应(PCR)检测支原体不仅可以确定属,还可以确定种。在本研究中,使用生化和分子方法对100份临床样本进行分析,以鉴定人型支原体、生殖支原体、微小脲原体和解脲脲原体。所呈现的结果证实了生化试验的低特异性以及使用分子方法对各个物种的检测效率更高。