The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, PR China.
Jiangsu Yuanyuan Bioengineering Co. Ltd, Xuzhou, Jiangsu, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1882-1887. doi: 10.1099/ijsem.0.003989.
A Gram-stain-positive, aerobic actinomycete, designated strain BMP B8144, was isolated from desert soil, in Xinjiang province, northwest China. The isolate produced scanty aerial mycelium and fragmented substrate mycelium on most tested media. Cell-wall hydrolysates contained -diaminopimelic acid, galactose and mannose. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylhydroxylethanolamine, phosphatidylinositol, and phosphatidylinositol mannosides. The major fatty acids included -C, C 8 and -C. The predominant menaquinones were MK-9(H) and MK-10(H). The DNA G+C content was 70.4 mol% (genome). Based on the 16S rRNA gene sequence analysis on EzBioCloud server, strain BMP B8144 showed the closest similarities to YIM LPA2h (98.9 %) and '' Hhs.015 (98.6 %). However, it can be distinguished from the closest strains based on the low levels of DNA-DNA relatedness (59.3±1.8 and 47.9±2.3 %, respectively). A combination of morphological, chemotaxonomic and phylogenetic characteristics, strain BMP B8144 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BMP B8144 (=CGMCC 4.7490=KCTC 49001).
一株革兰氏阳性需氧放线菌,被命名为 BMP B8144 菌株,从中国西北部新疆的沙漠土壤中分离得到。该分离株在大多数测试的培养基上产生少量气生菌丝和断裂的基质菌丝。细胞壁水解产物含有内消旋二氨基庚二酸、半乳糖和甘露糖。诊断性磷脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰羟乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要脂肪酸包括 C 16:0、C 8 和 C 18:0。主要的menaquinones 为 MK-9(H)和 MK-10(H)。DNA G+C 含量为 70.4 mol%(基因组)。基于 EzBioCloud 服务器上的 16S rRNA 基因序列分析,BMP B8144 株与 YIM LPA2h(98.9%)和 '' Hhs.015(98.6%)最为相似。然而,它可以通过与最接近的菌株之间较低的 DNA-DNA 同源性(分别为 59.3±1.8%和 47.9±2.3%)来区分。根据形态学、化学分类学和系统发育特征,BMP B8144 株代表了一个新的属,建议命名为 sp. nov. 。模式菌株为 BMP B8144(=CGMCC 4.7490=KCTC 49001)。