采用平行面对电极的电化学阻抗谱评估电极覆盖率的仿真与实验

Simulation and Experiment for Electrode Coverage Evaluation by Electrochemical Impedance Spectroscopy Using Parallel Facing Electrodes.

机构信息

Department of Electrical Systems, Graduate School of Science and Engineering, Ritsumeikan University.

Department of Electronic Engineering, Tohoku University.

出版信息

Anal Sci. 2020 Jul 10;36(7):853-858. doi: 10.2116/analsci.19P451. Epub 2020 Jan 24.

DOI:10.2116/analsci.19P451

PMID:31983717

Abstract

A parallel facing electrode (PFE) structure for adherent cell monitoring by electrochemical impedance spectroscopy (EIS) was developed, and its characteristics were investigated by both computer simulation and experiment. The PFE model consists of two facing gold electrode strips separated by 40 μm, and the area of its intersection is 500 × 500 μm. Computer simulation of EIS with adherent cells showed a distinct difference in solution resistance for different cell coverage, which was confirmed by experimental results using latex beads suspension. A well-defined relationship between solution resistance and cell coverage in our PFE is promising for quantitative evaluation of cell density, morphology and fatality.

摘要

开发了一种用于通过电化学阻抗谱（EIS）监测贴壁细胞的平行面对电极（PFE）结构，并通过计算机模拟和实验研究了其特性。PFE 模型由两条相互面对的金电极条组成，它们之间的间隔为 40μm，其交叉面积为 500×500μm。带有贴壁细胞的 EIS 的计算机模拟显示，不同细胞覆盖率的溶液电阻有明显差异，这一结果通过使用乳胶珠悬浮液的实验结果得到了证实。在我们的 PFE 中，溶液电阻与细胞覆盖率之间的明确关系有望用于细胞密度、形态和死亡率的定量评估。