基于金修饰 PDMS 基底的用于同时多重病原体检测的 SERS 适体传感器。

A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, China.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Apr 5;230:118103. doi: 10.1016/j.saa.2020.118103. Epub 2020 Jan 23.

DOI:10.1016/j.saa.2020.118103

PMID:32000058

Abstract

An aptamer-based sensitive method was developed here for detection of multiple foodborne pathogens in food matrix by surface-enhanced Raman scattering (SERS) technology. Polydimethylsiloxane (PDMS) film was first prepared and then coated with gold nanoparticles (AuNP) to act as an active substrate for the enhancement of Raman scattering. The as-prepared Au-PDMS film was functionalized with specific pathogen aptamers (Apt) to capture the targets. In addition, aptamers functionalized AuNP integrated with Raman reporters (4-Mercaptobenzoic acid (4-MBA)/Nile blue A (NBA)) were fabricated as pathogen-specific SERS probes. In this scheme, pathogens were first captured by Apt-Au-PDMS film and then bind with SERS probes to allow the formation of a sandwich assay to complete the sensor module for the detection of multiple pathogens. With Vibrio parahaemolyticus and Salmonella typhimurium as model targets, this protocol can selectively detect 18 cfu/mL and 27 cfu/mL, respectively. Furthermore, this platform can be successfully applied to detect pathogens in seafood samples with recoveries ranging from 82.9% to 95.1%.

摘要

这里开发了一种基于适体的敏感方法，通过表面增强拉曼散射（SERS）技术检测食品基质中的多种食源性致病菌。首先制备聚二甲基硅氧烷（PDMS）薄膜，然后用金纳米粒子（AuNP）涂覆，作为增强拉曼散射的活性基底。所制备的 Au-PDMS 薄膜用特定病原体适体（Apt）功能化，以捕获靶标。此外，将适体功能化的 AuNP 与拉曼报告分子（4-巯基苯甲酸（4-MBA）/尼罗蓝 A（NBA））集成，制成病原体特异性 SERS 探针。在该方案中，病原体首先被 Apt-Au-PDMS 薄膜捕获，然后与 SERS 探针结合，允许形成三明治测定法，完成用于检测多种病原体的传感器模块。以副溶血性弧菌和鼠伤寒沙门氏菌为模型靶标，该方案分别可选择性检测到 18 cfu/mL 和 27 cfu/mL。此外，该平台可成功应用于海鲜样品中病原体的检测，回收率范围为 82.9%至 95.1%。