单克隆抗体在磷脂朗缪尔-布洛杰特膜上的非均匀平移扩散。
Inhomogeneous translational diffusion of monoclonal antibodies on phospholipid Langmuir-Blodgett films.
作者信息
Wright L L, Palmer A G, Thompson N L
机构信息
Department of Physics, University of North Carolina, Chapel Hill 27599.
出版信息
Biophys J. 1988 Sep;54(3):463-70. doi: 10.1016/S0006-3495(88)82979-5.
Abstract
The translational mobility of fluorescent-labeled monoclonal antibodies specifically bound to supported phospholipid bilayers containing hapten-conjugated phospholipids has been measured as a function of the surface concentration of bound antibodies using fluorescence recovery after photobleaching. Fluorescence recovery curves are fit well by a model that assumes the presence of two populations of antibodies with different lateral diffusion coefficients. The larger diffusion coefficient equals 3.5 x 10(-9) cm2/s, the smaller diffusion coefficient ranges from 1.5 x 10(-9) cm2/s to 2.5 x 10(-10) cm2/s, and the fractional fluorescence recovery associated with the smaller coefficient increases from approximately 0 to approximately 0.7 with increasing concentration of bound antibody. These results suggest that complexes of haptenated phospholipids and antibodies in phospholipid Langmuir-Blodgett films form clusters or domains in a concentration-dependent fashion.
摘要
利用光漂白后的荧光恢复技术，测量了特异性结合含有半抗原共轭磷脂的支持磷脂双层的荧光标记单克隆抗体的平移流动性，该流动性是结合抗体表面浓度的函数。荧光恢复曲线能很好地拟合一个模型，该模型假定存在具有不同横向扩散系数的两种抗体群体。较大的扩散系数等于3.5×10⁻⁹ cm²/s，较小的扩散系数范围为1.5×10⁻⁹ cm²/s至2.5×10⁻¹⁰ cm²/s，并且随着结合抗体浓度的增加，与较小系数相关的荧光恢复分数从约0增加到约0.7。这些结果表明，磷脂朗缪尔-布洛杰特膜中半抗原化磷脂和抗体的复合物以浓度依赖的方式形成簇或结构域。
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本文引用的文献
1
Association dynamics and lateral transport in biological membranes.生物膜中的缔合动力学与侧向运输
J Supramol Struct Cell Biochem. 1981;17(1):61-7. doi: 10.1002/jsscb.380170107.
2
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Biochim Biophys Acta. 1982 Sep 13;721(1):30-8. doi: 10.1016/0167-4889(82)90020-9.
3
Two-dimensional crystallization technique for imaging macromolecules, with application to antigen--antibody--complement complexes.用于大分子成像的二维结晶技术及其在抗原-抗体-补体复合物中的应用。
Nature. 1983 Jan 13;301(5896):125-9. doi: 10.1038/301125a0.
4
Covalent linkage of a synthetic peptide to a fluorescent phospholipid and its incorporation into supported phospholipid monolayers.合成肽与荧光磷脂的共价连接及其掺入支持的磷脂单分子层中。
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5
Photon and fluorescence correlation spectroscopy and light scattering of eye-lens proteins at moderate concentrations.中等浓度下眼晶状体蛋白的光子与荧光相关光谱及光散射
Biophys J. 1983 Sep;43(3):345-54. doi: 10.1016/S0006-3495(83)84358-6.
6
Structural basis of antibody function.抗体功能的结构基础。
Annu Rev Immunol. 1983;1:87-117. doi: 10.1146/annurev.iy.01.040183.000511.
7
Protein diffusion in cell membranes: some biological implications.细胞膜中的蛋白质扩散：一些生物学意义。
Int Rev Cytol. 1984;87:19-81. doi: 10.1016/s0074-7696(08)62439-0.
8
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Drug Intell Clin Pharm. 1983 May;17(5):357-66. doi: 10.1177/106002808301700507.
9
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10
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J Biol Chem. 1982 Jun 10;257(11):6427-33.
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