补充精氨酸和鸟氨酸对高蛋白饮食成年猫某些细胞免疫变量的影响。

Effects of arginine and ornithine supplementation to a high-protein diet on selected cellular immune variables in adult cats.

机构信息

Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

Clinic for Small Animals, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

出版信息

J Vet Intern Med. 2020 Mar;34(2):852-856. doi: 10.1111/jvim.15736. Epub 2020 Feb 21.

DOI:10.1111/jvim.15736

PMID:32086838

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7096607/

Abstract

BACKGROUND

Dietary protein and amino acid intake and composition can modulate immune function.

OBJECTIVES

To evaluate the effects of high-protein intake and arginine and ornithine supplementation on feline immune cells.

ANIMALS

Ten healthy cats.

METHODS

Experimental study. Cats received a high-protein basal diet as a single daily meal. A crossover design was applied with treatments being basal diet (w/o); basal diet with arginine supplementation (+50, 75, 100% compared to the arginine provision by the basal diet; Arg 1-3); and basal diet with ornithine supplementation (+100, 150, 200% compared to the arginine provision by the basal diet; Orn 1-3). Blood samples were collected at the end of each 11-day treatment period.

RESULTS

Mitogen-stimulated proliferative activity of blood leukocytes revealed a quadratic effect for the dietary supplementation of arginine (P = .02) and ornithine (P = .03) (means for ConA-stimulation: w/o = 6.96; Arg 1 = 9.31; Arg 2 = 11.4; Arg 3 = 8.04; Orn 1 = 15.4; Orn 2 = 9.43; Orn 3 = 9.28; pooled SEM: 0.96). The number (% gated) of phagocytic granulocytes linearly decreased with increasing dietary concentrations of arginine (P = .05) and ornithine (P = .03) (means: w/o = 95.5; Arg 1 = 93.0; Arg 2 = 92.5; Arg 3 = 92.6; Orn 1 = 92.6; Orn 2 = 92.6; Orn 3 = 91.5; pooled SEM = 0.44).

CONCLUSIONS AND CLINICAL IMPORTANCE

This study could demonstrate immunomodulating properties of dietary arginine and ornithine in cats.

摘要

背景

膳食蛋白质和氨基酸的摄入和组成可以调节免疫功能。

目的

评估高蛋白摄入和精氨酸及瓜氨酸补充对猫免疫细胞的影响。

动物

10 只健康猫。

方法

实验研究。猫接受高蛋白基础日粮作为每日单餐。应用交叉设计，处理措施为基础日粮（无）；基础日粮加精氨酸补充（比基础日粮提供的精氨酸分别增加 50%、75%、100%；Arg1-3）；基础日粮加瓜氨酸补充（比基础日粮提供的精氨酸分别增加 100%、150%、200%；Orn1-3）。在每个 11 天治疗期结束时采集血样。

结果

血液白细胞的有丝分裂刺激增殖活性显示出日粮精氨酸（P=0.02）和瓜氨酸（P=0.03）补充的二次效应（ConA 刺激的平均值：无=6.96；Arg1=9.31；Arg2=11.4；Arg3=8.04；Orn1=15.4；Orn2=9.43；Orn3=9.28；合并 SEM：0.96）。随着日粮精氨酸（P=0.05）和瓜氨酸（P=0.03）浓度的增加，吞噬性粒细胞的数量（%门控）呈线性下降（平均值：无=95.5；Arg1=93.0；Arg2=92.5；Arg3=92.6；Orn1=92.6；Orn2=92.6；Orn3=91.5；合并 SEM=0.44）。