眼表外胚层在视网膜血管系统发育中的作用。 （你提供的原文“Role of from ocular surface ectoderm”似乎表述不完整，少了关键信息，但按照现有内容翻译如上）
Role of from ocular surface ectoderm in retinal vasculature development.
作者信息
Li Jing, Zhang Jin-Song, Zhao Jiang-Yue, Han Guo-Ge
机构信息
Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and Visual Science, Nankai University, Tianjin 300000, China.
Shenyang Aier Excellence Eye Hospital, Shenyang 110000, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2020 Feb 18;13(2):231-238. doi: 10.18240/ijo.2020.02.05. eCollection 2020.
AIM
To investigate how signals from lens regulate retinal vascular development and neovascularization.
METHODS
Le-Cre transgenic mouse line was employed to inactivate in the surface ectoderm selectively. Standard histological and whole-mount retina staining were employed to reveal morphological changes of retinal vasculature in defective eye. cDNA microarray and subsequent analyses were conducted to investigate the molecular mechanism underlying the vascular phenotype. Quantitative polymerase chain reaction (qPCR) was carried out to verify the microarrays results.
RESULTS
We found that inactivation of specifically on surface ectoderm leads to a variety of retinal vasculature anomalies. Microarray analyses and qPCR revealed that Sema3c, Sema3e, Nrp1, Tie1, Sox7, Sox17, and Sox18 are significantly affected in the knockout retinas at different developmental stages, suggesting that ocular surface ectoderm-derived Smad4 can signal to the retina and regulates various angiogenic signaling in the retina.
CONCLUSION
Our data suggest that the cross-talk between ocular surface ectoderm and retina is important for retinal vasculature development, and regulates various signaling associated with sprouting angiogenesis, vascular remodeling and maturation in the retina of mice.
目的
研究来自晶状体的信号如何调节视网膜血管发育和新生血管形成。
方法
采用Le-Cre转基因小鼠品系选择性地使表面外胚层中的[具体基因]失活。运用标准组织学和视网膜整体染色来揭示缺陷眼中视网膜血管系统的形态变化。进行cDNA微阵列及后续分析以探究血管表型背后的分子机制。开展定量聚合酶链反应（qPCR）以验证微阵列结果。
结果
我们发现特异性地在表面外胚层使[具体基因]失活会导致多种视网膜血管系统异常。微阵列分析和qPCR显示，在不同发育阶段，Sema3c, Sema3e, Nrp1, Tie1, Sox7, Sox17和Sox18在基因敲除的视网膜中受到显著影响，这表明眼表面外胚层来源的Smad4可向视网膜发出信号并调节视网膜中的各种血管生成信号。
结论
我们的数据表明，眼表面外胚层与视网膜之间的相互作用对于视网膜血管系统发育很重要，并且[具体基因]调节小鼠视网膜中与发芽血管生成、血管重塑和成熟相关的各种信号。
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