State Key Laboratory for Turbulence and Complex System and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing, 100871, PR China.
State Key Laboratory for Turbulence and Complex System and Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing, 100871, PR China.
Acta Biomater. 2020 Apr 1;106:396-409. doi: 10.1016/j.actbio.2020.02.024. Epub 2020 Feb 22.
Selection of an appropriate membrane material for guided bone regeneration (GBR) is still ongoing among resorbable and nonresorbable membranes with different characteristics. The major problem with nonresorbable membranes is the inevitable secondary surgery, while resorbable polymer membranes have limitations in providing sufficient mechanical support during the bone repair period due to premature loss of mechanical strength. Pure magnesium foil has been evaluated to explore its feasibility as a resorbable GBR membrane. It exhibited better mechanical properties, whereas poor formability and fast degradation rate were noted. In light of this, pure zinc membrane was developed as a pilot research in this paper. We designed three types of pure zinc membranes: pure Zn without pores, pure Zn with 300 µm diameter and 1000 µm diameter pores, and pure titanium without pores as a control. The mechanical property, in vitro immersion tests, and MC3T3-E1 cell viability assays were tested. Moreover, in vivo behaviors of three type zinc membranes were evaluated by using a rat calvarial critical-sized bone defect model. The experimental results indicated that pure Zn membrane with 300 µm pores showed the most favorable osteogenic capability, comparable to that of titanium membrane without pores. Therefore, considering appropriate degradation rate, adequate mechanical maintenance, and profitable osteogenic capacity, metallic pure zinc is believed to be a promising candidate for barrier membranes in GBR therapy for bone regeneration, and its mechanical property can be enhanced with further alloying. STATEMENT OF SIGNIFICANCE: Metallic element zinc plays a pivotal role in the growth and mineralization of bone tissues. As a pilot research, three type of guided bone regeneration (GBR) membranes were developed in the present work: pure Zn without pores, pure Zn with 300 µm-diameter and 1000 µm-diameter pores respectively. The mechanical property, in vitro immersion tests and MC3T3-E1 cell viability assays were tested, with pure titanium without pores as a control, thereafter the in vivo performance were evaluated by using a rat calvarial critical-sized bone defect model. It indicated that pure Zn membrane with 300 µm pores showed the most favorable osteogenic capability, comparable to that of titanium membrane control, and is believed to be a promising material candidate as barrier membrane in GBR therapy for bone regeneration.
用于引导骨再生（GBR）的合适膜材料的选择仍在继续，涉及具有不同特性的可吸收和不可吸收膜。不可吸收膜的主要问题是不可避免的二次手术，而可吸收聚合物膜由于机械强度过早丧失，在骨修复期间提供足够的机械支撑方面存在局限性。纯镁箔已被评估，以探索其作为可吸收 GBR 膜的可行性。它表现出更好的机械性能，但是延展性差和降解速度快。有鉴于此，本文开发了纯锌膜作为初步研究。我们设计了三种类型的纯锌膜：无孔纯锌、直径 300µm 和 1000µm 孔的纯锌以及无孔纯钛作为对照。测试了机械性能、体外浸泡试验和 MC3T3-E1 细胞活力测定。此外，通过使用大鼠颅骨临界尺寸骨缺损模型评估了三种类型锌膜的体内行为。实验结果表明，具有 300µm 孔的纯锌膜表现出最有利的成骨能力，可与无孔钛膜相媲美。因此，考虑到适当的降解率、足够的机械维持和有利的成骨能力，金属纯锌被认为是 GBR 治疗骨再生中屏障膜的有前途的候选材料，其机械性能可以通过进一步合金化来提高。
金属元素锌在骨骼组织的生长和矿化中起着关键作用。作为初步研究，本工作开发了三种类型的引导骨再生（GBR）膜：分别为无孔纯锌、直径 300µm 和 1000µm 孔的纯锌。测试了机械性能、体外浸泡试验和 MC3T3-E1 细胞活力测定，以无孔纯钛作为对照，然后通过使用大鼠颅骨临界尺寸骨缺损模型评估了体内性能。结果表明，具有 300µm 孔的纯锌膜表现出最有利的成骨能力，可与钛膜对照相媲美，被认为是作为 GBR 治疗骨再生中屏障膜的有前途的材料候选物。
