从[具体来源未给出]提取的粗多糖对小鼠四氯化碳诱导的肝损伤的保肝作用。
Hepatoprotective effect of crude polysaccharides extracted from against carbon tetrachloride-induced liver injury in mice.
作者信息
Susilo Raden Joko Kuncoroningrat, Winarni Dwi, Husen Saikhu Akhmad, Hayaza Suhailah, Punnapayak Hunsa, Wahyuningsih Sri Puji Astuti, Sajidah Elma Sakinatus, Darmanto Win
机构信息
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
Vet World. 2019 Dec;12(12):1987-1991. doi: 10.14202/vetworld.2019.1987-1991. Epub 2019 Dec 17.
BACKGROUND AND AIM
Natural products are currently widely used as alternative treatments for liver disease. The study aimed to determine the hepatoprotective effect of crude polysaccharides extracted from against liver injury induced by carbon tetrachloride (CCl).
MATERIALS AND METHODS
Twenty-four male BALB/C mice were randomly divided into six groups. Serum and liver samples were taken on day 10 after administration. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were measured using enzyme-linked immunosorbent assays, and the histology of the liver was evaluated using light microscopy.
RESULTS
extract significantly decreased the levels of ALT, AST, and MDA and significantly increased the levels of SOD and CAT. In the histological evaluation, the liver tissue of CCl-treated mice exhibited hydropic degeneration, necrosis, and sinusoidal dilatation. extract administration improved this liver tissue histopathology.
CONCLUSION
Crude polysaccharides extracted from showed a hepatoprotective effect, regenerating damaged liver tissue.
背景与目的
天然产物目前被广泛用作肝病的替代治疗方法。本研究旨在确定从[具体来源未给出]提取的粗多糖对四氯化碳（CCl）诱导的肝损伤的保肝作用。
材料与方法
将24只雄性BALB/C小鼠随机分为六组。给药后第10天采集血清和肝脏样本。使用酶联免疫吸附测定法测量丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平，并使用光学显微镜评估肝脏组织学。
结果
[提取物名称未给出]提取物显著降低了ALT、AST和MDA的水平，并显著提高了SOD和CAT的水平。在组织学评估中，CCl处理小鼠的肝组织表现出水样变性、坏死和窦状扩张。给予[提取物名称未给出]提取物改善了这种肝组织病理变化。
结论
从[具体来源未给出]提取的粗多糖显示出保肝作用，可使受损肝组织再生。
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