通过反向微乳液法制备磷酸钙/质粒DNA纳米颗粒：利用实验设计优化配方变量

Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design.

作者信息

Li Wenpan, Jing Shasha, Xin Xiu, Zhang Xirui, Chen Kang, Chen Dawei, Hu Haiyang

机构信息

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China.

出版信息

Asian J Pharm Sci. 2017 Mar;12(2):179-186. doi: 10.1016/j.ajps.2016.09.006. Epub 2016 Nov 4.

DOI:10.1016/j.ajps.2016.09.006

PMID:32104328

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7032106/

Abstract

In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box-Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 ± 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.

摘要

在本研究中，采用 Triton X - 100/丁醇/环己烷/水反相微乳液体系制备了磷酸钙/质粒 DNA 纳米颗粒。基于 Box - Behnken 设计方法对粒径的评估来优化制备条件。优化后的磷酸钙/质粒 DNA 纳米颗粒的粒径为 60.23 ± 4.72 nm，多分散指数为 0.252，质粒 DNA 包封效率超过 90%。优化后的磷酸钙/质粒 DNA 纳米颗粒具有 pH 敏感性和生物相容性。此外，优化后的磷酸钙/质粒 DNA 纳米颗粒显示出更高的转染效率。