研究 smad 家族成员 7 在口腔黏膜下纤维性变和口腔鳞状细胞癌中的表达和功能。

Study on the expression and function of smad family member 7 in oral submucous fibrosis and oral squamous cell carcinoma.

机构信息

Oral Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410000, China.

Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan 410000, China.

出版信息

Arch Oral Biol. 2020 Apr;112:104687. doi: 10.1016/j.archoralbio.2020.104687. Epub 2020 Feb 24.

DOI:10.1016/j.archoralbio.2020.104687

PMID:32114253

Abstract

OBJECTIVE

To investigate the expression and function of smad family member 7 (SMAD7) in the progress of oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC).

METHODS

Mucosa tissue microarray containing 12 normal oral mucosa samples, 69 OSF samples, 28 OSCC sample and paired adjacent tissues was used to explore the expression levels in OSF and OSCC by immunochemistry. Several online bioinformatics analysis tools were used to explore "transcriptome level" and mostly probable "functions and pathways" of SMAD7 in head and neck squamous cell carcinoma.

RESULTS

SMAD7 expression was up-regulated significantly in "OSF" (P < 0.0001) and "OSCC" (P < 0.05). In the status of "OSF and OSCC with OSF", the trends of SMAD7 expression were consistent and up-regulated (P < 0.0001). Based on bioinformatics analysis results, SMAD7 was significantly higher in head and neck squamous cell carcinoma than that of normal tissues. No mutation was found in head and neck squamous cell carcinoma. Pathway analysis results showed three mostly probable functions (extracellular matrix organization, blood vessel development and laminin interactions) and two mostly probable pathways (regulation of actin cytoskeleton and ras-associated protein-1 signaling pathway) that SMAD7 participated in.

CONCLUSIONS

In OSF and OSCC, SMAD7 is indicated to be a promoter, as well as a potential diagnostic biomarker.

摘要

目的

研究 SMAD 家族成员 7（SMAD7）在口腔黏膜下纤维化（OSF）和口腔鳞状细胞癌（OSCC）进展中的表达和功能。

方法

使用包含 12 例正常口腔黏膜组织、69 例 OSF 组织、28 例 OSCC 组织及配对的相邻组织的黏膜组织微阵列，通过免疫组织化学方法探讨 SMAD7 在 OSF 和 OSCC 中的表达水平。利用多个在线生物信息学分析工具，探讨 SMAD7 在头颈部鳞状细胞癌中的“转录组水平”和最可能的“功能和途径”。

结果

SMAD7 在“OSF”（P<0.0001）和“OSCC”（P<0.05）中的表达明显上调。在“OSF 和 OSCC 伴 OSF”状态下，SMAD7 的表达趋势一致且上调（P<0.0001）。基于生物信息学分析结果，SMAD7 在头颈部鳞状细胞癌中的表达明显高于正常组织。在头颈部鳞状细胞癌中未发现突变。通路分析结果显示 SMAD7 参与了三个最可能的功能（细胞外基质组织、血管发育和层粘连蛋白相互作用）和两个最可能的途径（肌动蛋白细胞骨架的调节和 ras 相关蛋白-1 信号通路）。