纳米铜中毒对乌蒙半细毛羊免疫和抗氧化功能的影响
Effects of Nano-Copper Poisoning on Immune and Antioxidant Function in the Wumeng Semi-Fine Wool Sheep.
作者信息
Song ChunJie, Gan Shangquan, Shen Xiaoyun
机构信息
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, Xinjiang, 832000, China.
出版信息
Biol Trace Elem Res. 2020 Dec;198(2):515-520. doi: 10.1007/s12011-020-02085-z. Epub 2020 Mar 4.
The Wumeng semi-fine wool sheep is vital to the production system of the Wumeng mountainous area in Southwest China. To study the effect of nano-copper poisoning in the Wumeng semi-fine wool sheep, nano-copper poisoning model was established in sheep. We determined blood physiological and biochemical parameters, immune indexes, and antioxidant indicators. The results showed that Hb, RBC, and PCV levels in blood from the nano-copper group were markedly lower (P < 0.01) than those in the control group. Serum LDH, AST, ALT, CPK, and Cp from the nano-copper group were significantly higher (P < 0.01) than those in control animals. Serum SOD, GSH-Px, CAT, and T-AOC from the nano-copper group were significantly lower (P < 0.01) than those in control sheep, and MDA content in serum from the nano-copper group were markedly higher (P < 0.01) than those in control sheep. The levels of IL-2, IL-6, IL-1β, IgA, IgM, and IgG from the nano-copper group were significantly lower (P < 0.01) than those in the control group. It was concluded that nano-copper poisoning could not only affect the immune function of Wumeng semi-fine wool sheep but also reduce the antioxidant capacity.
乌蒙半细毛羊对中国西南部乌蒙山区的生产系统至关重要。为研究纳米铜中毒对乌蒙半细毛羊的影响，在绵羊中建立了纳米铜中毒模型。我们测定了血液生理生化参数、免疫指标和抗氧化指标。结果显示，纳米铜组血液中的血红蛋白（Hb）、红细胞（RBC）和红细胞压积（PCV）水平显著低于对照组（P<0.01）。纳米铜组血清中的乳酸脱氢酶（LDH）、谷草转氨酶（AST）、谷丙转氨酶（ALT）、肌酸磷酸激酶（CPK）和铜蓝蛋白（Cp）显著高于对照动物（P<0.01）。纳米铜组血清中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）显著低于对照绵羊（P<0.01），纳米铜组血清中的丙二醛（MDA）含量显著高于对照绵羊（P<0.01）。纳米铜组白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、免疫球蛋白A（IgA）、免疫球蛋白M（IgM）和免疫球蛋白G（IgG）水平显著低于对照组（P<0.01）。研究得出结论，纳米铜中毒不仅会影响乌蒙半细毛羊的免疫功能，还会降低其抗氧化能力。
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