Université de Nantes, CNRS, CEISAM UMR 6230, F-44000 Nantes, France.
Analyst. 2020 Apr 7;145(7):2457-2472. doi: 10.1039/d0an00142b. Epub 2020 Mar 10.
NMR spectroscopy is an essential analytical technique in metabolomics and fluxomics workflows, owing to its high structural elucidation capabilities combined with its intrinsic quantitative nature. However, routine NMR "omic" analytical methods suffer from several drawbacks that may have limited their use as a method of choice, in particular when compared to another widely used technique, mass spectrometry. This review describes, in a critical and perspective discussion, how some of the most recent developments emerging from the NMR community could act as real game changers for metabolomics and fluxomics in the near future. Advanced developments to make NMR metabolomics more resolutive, more sensitive and more accessible are described, as well as new approaches to improve the identification of biomarkers. We hope that this review will convince a broad end-user community of the increasing role of NMR in the "omic" world at the beginning of the 2020s.
NMR 光谱学是代谢组学和通量组学工作流程中的一种重要分析技术，这归因于其高结构解析能力以及固有的定量性质。然而，常规的 NMR“组学”分析方法存在一些缺点，这可能限制了它们作为首选方法的使用，特别是与另一种广泛使用的技术——质谱法相比。本文以批判性和前瞻性的讨论方式描述了 NMR 领域的一些最新进展，这些进展可能会在不久的将来成为代谢组学和通量组学的真正变革者。本文还描述了使 NMR 代谢组学更具分辨率、更灵敏和更易获得的先进发展，以及改进生物标志物鉴定的新方法。我们希望，这篇综述将说服广大的终端用户群体在 21 世纪初认识到 NMR 在“组学”世界中的作用越来越大。
