非阻塞性无精子症患者的长非编码 RNA 表达谱和长非编码 RNA 介导的竞争性内源性 RNA 网络。
Long noncoding RNAs expression profile and long noncoding RNA-mediated competing endogenous RNA network in nonobstructive azoospermia patients.
机构信息
Department of Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, PR China.
Key Laboratory of Human Stem Cells and Reproductive of the Ministry of Health, Institute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, PR China.
出版信息
Epigenomics. 2020 Apr;12(8):673-684. doi: 10.2217/epi-2020-0008. Epub 2020 Mar 16.
To analyze the expression profile and competing endogenous RNA (ceRNA) network of long noncoding RNAs (lncRNAs) in nonobstructive azoospermia (NOA). The lncRNA expression profile in NOA was determined by microarray reanalysis. Differential expression analysis was performed by R software. The ceRNA network was constructed using correlation analysis and gene target miRNA prediction. Metascape was used for enrichment analysis. Again ceRNA network was validated by quantitative real-time PCR. Many lncRNAs are differently expressed in NOA. LncRNAs might participate in spermatogenesis through ceRNA mechanism. The ceRNA network included male gamete generation and other pathways. LINC00467 in the network regulated the expression of and . LncRNAs are involved in NOA and potential biomarkers and therapeutic targets for NOA.
分析非梗阻性无精子症（NOA）中长链非编码 RNA（lncRNAs）的表达谱和竞争内源性 RNA（ceRNA）网络。通过微阵列重新分析确定 NOA 中的 lncRNA 表达谱。使用 R 软件进行差异表达分析。使用相关分析和基因靶 miRNA 预测构建 ceRNA 网络。Metascape 用于富集分析。再次通过定量实时 PCR 验证 ceRNA 网络。NOA 中许多 lncRNAs 的表达水平存在差异。lncRNAs 可能通过 ceRNA 机制参与精子发生。ceRNA 网络包括精子发生和其他途径。网络中的 LINC00467 调节 和 的表达。lncRNAs 参与 NOA，是潜在的生物标志物和治疗靶点。
Zotero 插件