Wang Hui, He Zixu, Yang Yuyun, Zhang Jiao, Zhang Wei, Zhang Wen, Li Ping, Tang Bo
College of Chemistry , Chemical Engineering and Materials Science , Institute of Biomedical Sciences , Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong , Key Laboratory of Molecular and Nano Probes , Ministry of Education , Shandong Normal University , Jinan 250014 , PR China . Email:
Chem Sci. 2019 Oct 7;10(47):10876-10880. doi: 10.1039/c9sc04384e. eCollection 2019 Dec 21.
Golgi oxidative stress is significantly associated with the occurrence and progression of hypertension. Notably, the concentration of hydrogen peroxide (HO) is directly proportional to the degree of Golgi oxidative stress. Therefore, based on a novel Golgi-targeting phenylsulfonamide group, we developed a two-photon (TP) fluorescent probe, Np-Golgi, for HO ratiometric imaging in living systems. The phenylsulfonamide moiety effectively assists Np-Golgi in the precise location of Golgi apparatus. In addition, the raw material of phenylsulfonamide is easily available, and chemical modification is easily implemented. By application of Np-Golgi, we explored the generation of HO during Golgi oxidative stress, and also successfully revealed increases on the levels of Golgi HO in the kidneys of mice with hypertension. This work provides an ideal tool to monitor Golgi oxidative stress for the first time and novel drug targets for the future treatment of hypertension.
高尔基体氧化应激与高血压的发生和发展显著相关。值得注意的是，过氧化氢（HO）的浓度与高尔基体氧化应激程度成正比。因此，基于一种新型的靶向高尔基体的苯磺酰胺基团，我们开发了一种双光子（TP）荧光探针Np-Golgi，用于在活体系统中对HO进行比率成像。苯磺酰胺部分有效地帮助Np-Golgi精确靶向高尔基体。此外，苯磺酰胺的原料易于获得，且易于进行化学修饰。通过应用Np-Golgi，我们探究了高尔基体氧化应激过程中HO的产生情况，并且成功揭示了高血压小鼠肾脏中高尔基体HO水平的升高。这项工作首次提供了监测高尔基体氧化应激的理想工具以及未来治疗高血压的新药物靶点。
Zotero 插件
