一种用于肿瘤细胞迁移三维分析的新型工作流程。
A novel workflow for three-dimensional analysis of tumour cell migration.
作者信息
Ketchen Sophie, Rohwedder Arndt, Knipp Sabine, Esteves Filomena, Struve Nina, Peckham Michelle, Ladbury John E, Curd Alistair, Short Susan C, Brüning-Richardson Anke
机构信息
Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, UK.
School of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
出版信息
Interface Focus. 2020 Apr 6;10(2):20190070. doi: 10.1098/rsfs.2019.0070. Epub 2020 Feb 14.
Abstract
The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
摘要
二维分析在三维（3D）细胞成像中的局限性损害了生物学研究中研究结果的准确性。在此，我们报告一种用于获取、分析和解释肿瘤球体图像的新型3D方法。我们对间充质-阿米巴样转变的研究兴趣促成了一个工作流程的开发，该流程将3D数据的生成和分析与即时结构照明显微镜及一个新的ImageJ插件相结合。
相似文献
1
A novel workflow for three-dimensional analysis of tumour cell migration.
Interface Focus. 2020 Apr 6;10(2):20190070. doi: 10.1098/rsfs.2019.0070. Epub 2020 Feb 14.
2
LSM-W: laser scanning microscopy worker for wheat leaf surface morphology.
BMC Syst Biol. 2019 Mar 5;13(Suppl 1):22. doi: 10.1186/s12918-019-0689-8.
3
Quantitative 3D structured illumination microscopy of nuclear structures.
Nat Protoc. 2017 May;12(5):1011-1028. doi: 10.1038/nprot.2017.020. Epub 2017 Apr 13.
4
A workflow for 3D-CLEM investigating liver tissue.
J Microsc. 2021 Mar;281(3):231-242. doi: 10.1111/jmi.12967. Epub 2020 Oct 27.
5
'Cloudbuster': a Python-based open source application for three-dimensional reconstruction and quantification of stacked biological imaging samples.
Interface Focus. 2022 Aug 12;12(5):20220016. doi: 10.1098/rsfs.2022.0016. eCollection 2022 Oct 6.
6
QSIM: quantitative structured illumination microscopy image processing in ImageJ.
Biomed Eng Online. 2015 Jan 14;14:4. doi: 10.1186/1475-925X-14-4.
7
TACI: An ImageJ Plugin for 3D Calcium Imaging Analysis.
J Vis Exp. 2022 Dec 16(190). doi: 10.3791/64953.
8
The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures.
Sci Data. 2025 Feb 17;12(1):283. doi: 10.1038/s41597-025-04441-x.
9
A novel 3D spheroid model of rheumatoid arthritis synovial tissue incorporating fibroblasts, endothelial cells, and macrophages.
Front Immunol. 2023 Jul 20;14:1188835. doi: 10.3389/fimmu.2023.1188835. eCollection 2023.
10
Ready to go 3D? A semi-automated protocol for microwell spheroid arrays to increase scalability and throughput of 3D cell culture testing.
Toxicol Mech Methods. 2020 Oct;30(8):590-604. doi: 10.1080/15376516.2020.1800881. Epub 2020 Aug 26.
引用本文的文献
1
'Cloudbuster': a Python-based open source application for three-dimensional reconstruction and quantification of stacked biological imaging samples.
Interface Focus. 2022 Aug 12;12(5):20220016. doi: 10.1098/rsfs.2022.0016. eCollection 2022 Oct 6.
2
Drug Resistance in Glioma Cells Induced by a Mesenchymal-Amoeboid Migratory Switch.
Biomedicines. 2021 Dec 22;10(1):9. doi: 10.3390/biomedicines10010009.
3
Composition of receptor tyrosine kinase-mediated lipid micro-domains controlled by adaptor protein interaction.
Sci Rep. 2021 Mar 17;11(1):6160. doi: 10.1038/s41598-021-85578-8.
本文引用的文献
1
Factors associated with failure of oncology drugs in late-stage clinical development: A systematic review.
Cancer Treat Rev. 2017 Jan;52:12-21. doi: 10.1016/j.ctrv.2016.10.009. Epub 2016 Nov 4.
2
High-content analysis of tumour cell invasion in three-dimensional spheroid assays.
Oncoscience. 2015 Jun 14;2(6):596-606. doi: 10.18632/oncoscience.171. eCollection 2015.
3
Construction of an instant structured illumination microscope.
Methods. 2015 Oct 15;88:37-47. doi: 10.1016/j.ymeth.2015.07.012. Epub 2015 Jul 22.
4
Instant super-resolution imaging in live cells and embryos via analog image processing.
Nat Methods. 2013 Nov;10(11):1122-6. doi: 10.1038/nmeth.2687. Epub 2013 Oct 6.
5
Current challenges in clinical target volume definition: tumour margins and microscopic extensions.
Acta Oncol. 2012 Nov;51(8):984-95. doi: 10.3109/0284186X.2012.720381. Epub 2012 Sep 24.
6
In vitro evaluation of clinical activity and toxicity of anticancer drugs using tumor cells from patients and cells representing normal tissues.
Cancer Chemother Pharmacol. 2012 Mar;69(3):697-707. doi: 10.1007/s00280-011-1746-1. Epub 2011 Oct 9.
7
Cell migration: Moving in 3D.
Nat Rev Mol Cell Biol. 2010 Jul;11(7):465. doi: 10.1038/nrm2925. Epub 2010 Jun 9.
8
The molecular mechanisms of transition between mesenchymal and amoeboid invasiveness in tumor cells.
Cell Mol Life Sci. 2010 Jan;67(1):63-71. doi: 10.1007/s00018-009-0132-1. Epub 2009 Aug 26.
9
Filopodia: molecular architecture and cellular functions.
Nat Rev Mol Cell Biol. 2008 Jun;9(6):446-54. doi: 10.1038/nrm2406. Epub 2008 May 9.
10
CCG-1423: a small-molecule inhibitor of RhoA transcriptional signaling.
Mol Cancer Ther. 2007 Aug;6(8):2249-60. doi: 10.1158/1535-7163.MCT-06-0782.
Zotero 插件