[巴戟颗粒改善雷公藤多苷诱导的少弱精子症大鼠的精子质量]

[Bazi Granules improves sperm quality in rats with oligoasthenozoospermia induced by multi-glycosides of tripterygium wilfordii].

作者信息

Tan Tian-Yang, Zheng Yan-Fei, Tang Yi-Bo, Bai Xue, Liu Mei-Qi, Li Yue, Li Yun-Xin, Liu Zhen-Quan, Wang Qi

机构信息

School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

出版信息

Zhonghua Nan Ke Xue. 2018 Nov;24(11):1016-1020.

PMID:32212477

Abstract

OBJECTIVE

To investigate the effect of Bazi Granules on sperm quality in male rats with oligoasthenozoospermia (OAS) induced by multi-glycosides of tripterygium wilfordii (GTW).

METHODS

Thirty-six SD male rats were randomly divided into six groups of equal number: normal control, OAS model control, Wuziyanzong Pills (WYP), and low-, medium- and high-dose Bazi. The OAS model was established in the rats except those of the normal control group by intragastrical delivery of GTW at 30 mg/kg/d for 40 days. From the 41st day, the animals of the normal and OAS model control groups were fed with distilled water, those of the WYP group treated by gavage with WYP at 1.02 g/kg/d, and those of the low-, medium- and high-dose Bazi groups intragastically given Bazi Granules 3 (5.27 g/kg), 6 (10.54 g/kg) and 12 (21.08 g/kg) times, respectively, that of the human-equivalent dose. Semen parameters and the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in the testis tissue were determined after 28 days of treatment.

RESULTS

After treatment, the rats of the of the high-, medium- and low-dose Bazi groups, compared with the OAS model controls, showed significant increases in sperm concentration (［1 050.71 ± 203.71］, ［1 370.06 ± 166.01］ and ［1 302.53 ± 476.51］ vs ［617.01 ± 237.08］ ×10⁶/ml, P < 0.05), sperm motility (［0.56 ± 0.24］%, ［0.73 ± 0.14］% and ［0.70 ± 0.23］% vs ［0.07 ± 0.05］%, P < 0.05), sperm average path velocity (［85.71 ± 30.35］, ［83.83 ± 10.31］ and ［75.06 ± 19.70］ vs ［43.45 ± 38.74］ μm/s, P < 0.05), sperm curvilinear velocity (［101.76 ± 23.28］, ［119.60 ± 21.22］ and ［102.11 ± 32.89］ vs ［53.63 ± 47.91］ μm/s, P < 0.05), sperm straight line velocity (［62.75 ± 7.63］, ［67.80 ± 5.05］ and ［64.11 ± 12.03］ vs ［40.18 ± 36.86］ μm/s, P < 0.05), and the SOD level (［380.23 ± 75.07］, ［349.53 ± 97.48］ and ［415.07 ± 72.01］ vs ［304.62 ± 27.17］ U/mg, P < 0.05), but a remarkable decrease in the MDA level (［0.33 ± 0.16］, ［0.22 ± 0.05］ and ［0.34 ± 0.22］ vs ［0.73 ± 0.20］ nmol/mg, P < 0.05).

CONCLUSIONS

Bazi Granules can significantly improve the sperm quality of OAS rats, which may be related to its abilities of repairing oxidative stress injury and enhancing oxidation resistance.

摘要

目的

探讨八子颗粒对雷公藤多苷（GTW）诱导的少弱精子症（OAS）雄性大鼠精子质量的影响。

方法

将36只SD雄性大鼠随机分为6组，每组数量相等：正常对照组、OAS模型对照组、五子衍宗丸（WYP）组以及低、中、高剂量八子组。除正常对照组大鼠外，其余大鼠通过灌胃给予30 mg/kg/d的GTW建立OAS模型，持续40天。从第41天起，正常对照组和OAS模型对照组大鼠给予蒸馏水，WYP组大鼠按1.02 g/kg/d的剂量灌胃给予WYP，低、中、高剂量八子组大鼠分别按相当于人等效剂量的3倍（5.27 g/kg）、6倍（10.54 g/kg）和12倍（21.08 g/kg）灌胃给予八子颗粒。治疗28天后，测定精液参数以及睾丸组织中超氧化物歧化酶（SOD）和丙二醛（MDA）的含量。

结果

治疗后，与OAS模型对照组相比，高、中、低剂量八子组大鼠的精子浓度（［1 050.71 ± 203.71］、［1 370.06 ± 166.01］和［1 302.53 ± 476.51］对［617.01 ± 237.08］×10⁶/ml，P < 0.05）、精子活力（［0.56 ± 0.24］%、［0.73 ± 0.14］%和［0.70 ± 0.23］%对［0.07 ± 0.05］%，P < 0.05）、精子平均路径速度（［85.71 ± 30.35］、［83.83 ± 10.31］和［75.06 ± 19.70］对［43.45 ± 38.74］μm/s，P < 0.05）、精子曲线速度（［101.76 ± 23.28］、［119.60 ± 21.22］和［102.11 ± 32.89］对［53.63 ± 47.91］μm/s，P < 0.05）、精子直线速度（［62.75 ± 7.63］、［67.80 ± 5.05］和［64.11 ± 12.03］对［40.18 ± 36.86］μm/s，P < 0.05）以及SOD水平（［380.23 ± 75.07］、［349.53 ± 97.48］和［415.07 ± 72.01］对［304.62 ± 27.17］U/mg，P < 0.05）均显著升高，而MDA水平显著降低（［0.33 ± 0.16］、［0.22 ± 0.05］和［0.34 ± 0.22］对［0.73 ± 0.20］nmol/mg，P < 0.05）。