白光和红光 LED 作为两相分批培养用于蓝藻色素生产。

White and red LEDs as two-phase batch for cyanobacterial pigments production.

机构信息

CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Novo Edifício do Terminal de Cruzeiros de Leixões, Avenida General Norton de Matos, s/n, 4450-208 Matosinhos, Portugal; FCUP - Faculty of Science, University of Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.

出版信息

Bioresour Technol. 2020 Jul;307:123105. doi: 10.1016/j.biortech.2020.123105. Epub 2020 Feb 29.

DOI:10.1016/j.biortech.2020.123105

PMID:32222686

Abstract

Carotenoids and phycobiliproteins have a high economic value, due to their wide range of biological and industrial applications. The implementation of strategies to increase their production, such as the application of two-phase light cultivation systems, can stimulate pigments production, increasing economic turnover. In this sense, Cyanobium sp. was grown in seven different two-phase white/red cultivation arrangements, varying the time of each light from 0 to 21 days. Biomass, photosynthetic activity, pigments profile and antioxidant capacity were measured along time. Red light increased photosynthetic activity and pigments content (ca. 1.8-fold), and the use of a two-phase cultivation system generally raised bioactivity and production of phytochemicals. Among the studied, the optimal cultivation condition was found with 10 days of white followed by 4 days of red light. The optimized growth led to a productivity of 137.4 ± 0.8 mg L d of biomass, 17.0 ± 0.2 mg L d of total phycobiliproteins and 4.5 ± 0.2 mg L d of carotenoids.

摘要

类胡萝卜素和藻胆蛋白具有很高的经济价值，因为它们具有广泛的生物和工业应用。实施增加其产量的策略，例如应用两相光培养系统，可以刺激色素的生产，增加经济收入。在这种意义上，在七种不同的两相白光/红光培养布置中培养了蓝藻 sp.，每种光的时间从 0 到 21 天不等。随着时间的推移，测量了生物量、光合作用活性、色素谱和抗氧化能力。红光增加了光合作用活性和色素含量（约 1.8 倍），并且两相培养系统的使用通常提高了生物活性和植物化学物质的生产。在所研究的条件中，发现使用 10 天的白光 followed by 4 天的红光的最佳培养条件。优化生长导致生物量的生产率为 137.4 ± 0.8 mg L d，总藻胆蛋白为 17.0 ± 0.2 mg L d 和类胡萝卜素为 4.5 ± 0.2 mg L d。